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首页> 外文期刊>Proteomics >Proteome man of normal rat retina and comparison with the proteome of diabetic rat retina: New insight in the pathogenesis of diabetic retinopathy
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Proteome man of normal rat retina and comparison with the proteome of diabetic rat retina: New insight in the pathogenesis of diabetic retinopathy

机译：正常大鼠视网膜的蛋白质组人与糖尿病大鼠视网膜的蛋白质组比较：糖尿病视网膜病变发病机理的新见解

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摘要

We have employed proteomics to establish a proteome map of the normal rat retina. This baseline map was then used for comparison with the early diabetic rat retinal proteome. Diabetic rat retinae were obtained from Dark Agouti rats after 10 wk of streptozotocin-induced hyperglycaemia. Extracted proteins from normal and diabetic rat retinae were separated and compared using 2-DE. A total of 145 protein spots were identified in the normal rat retina using MALDI-MS and database matching. LC-coupled ESI-MS increased the repertoire of identified proteins by 23 from 145 to 168. Comparison with early diabetic rat retinae revealed 24 proteins unique to the diabetic gels, and 37 proteins absent from diabetic gels. Uniquely expressed proteins identified included the HSPs 70.1A and 8, and platelet activating factor. There were eight spots with increased expression and 27 with decreased expression on diabetic gels. Beta catenin, phosducin and aldehyde reductase were increased in expression in diabetes whilst succinyl coA ligase and dihydropyrimidase-related protein were decreased. identification of such changes in protein expression has given new insights and a more comprehensive understanding of the pathogenesis of diabetic retinopathy, widening the scope of potential avenues for new therapies for this common cause of blindness.
机译：我们已经使用蛋白质组学来建立正常大鼠视网膜的蛋白质组图。然后将该基线图用于与早期糖尿病大鼠视网膜蛋白质组进行比较。糖尿病大鼠视网膜是在10周链脲佐菌素诱导的高血糖症后从Dark Agouti大鼠获得的。分离并从正常和糖尿病大鼠视网膜中提取蛋白质，并使用2-DE比较。使用MALDI-MS和数据库匹配，在正常大鼠视网膜中总共鉴定出145个蛋白斑点。 LC偶联的ESI-MS将鉴定出的蛋白质种类从145个增加到168个，增加了168个到168个。与早期糖尿病大鼠视网膜的比较显示，糖尿病凝胶具有24种独特的蛋白质，而糖尿病凝胶则缺乏37种蛋白质。鉴定出的独特表达的蛋白质包括HSP 70.1A和8，以及血小板活化因子。在糖尿病凝胶上有8个表达增加的斑点和27个表达减少的斑点。在糖尿病患者中，β-连环蛋白，光导蛋白和醛还原酶的表达增加，而琥珀酰辅酶A连接酶和二氢嘧啶酶相关的蛋白减少。蛋白质表达的这种变化的鉴定为糖尿病性视网膜病的发病机理提供了新的见识和更全面的理解，从而扩大了针对这种常见失明原因的新疗法的潜在途径的范围。

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