A method for the rapid depletion of albumin and immunoglobulin from human plasma

Greenough C; Jenkins RE; Kitteringham NR; Pirmohamed M; Park BK; Pennington SR

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A method for the rapid depletion of albumin and immunoglobulin from human plasma

机译：一种从人血浆中快速消耗白蛋白和免疫球蛋白的方法

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In studies of the plasma proteome, the high abundance of proteins such as albumin and immunoglobulin impedes the investigation of lower abundance proteins that may be more suitable as biomarkers of disease. We report the specific removal of 98% of albumin and 80% of immunoglobulin heavy chain from human plasma by affinity chromatography, and the subsequent improvement in the number of spots detected and their resolution following two-dimensional gel electrophoresis.

机译：在血浆蛋白质组学的研究中，诸如白蛋白和免疫球蛋白之类的蛋白质的高丰度阻碍了对较低丰度蛋白质的研究，这些蛋白质可能更适合作为疾病的生物标记。我们报告通过亲和色谱从人血浆中特异性清除98％的白蛋白和80％的免疫球蛋白重链，以及随后在二维凝胶电泳中检测到的斑点数量及其分辨率的改善。

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《Proteomics》 |2004年第10期|共5页
作者
Greenough C; Jenkins RE; Kitteringham NR; Pirmohamed M; Park BK; Pennington SR;
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正文语种 eng
中图分类分子生物学;
关键词
albumin; depletion; immunoglobulin; plasma; 2-DIMENSIONAL GEL-ELECTROPHORESIS; S-TRANSFERASE-PI; AFFINITY-CHROMATOGRAPHY; MASS-SPECTROMETRY; GAMMA-GLOBULIN; HUMAN SERUM; PROTEIN; BINDING; PURIFICATION; MICE;

机译：白蛋白;耗竭;免疫球蛋白;血浆;二维凝胶电泳;S-转移酶-PI;亲和层析;质谱法;γ-球蛋白;人血清;蛋白质;结合;纯化;小鼠;

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1. A method for the rapid depletion of albumin and immunoglobulin from human plasma [J] . Greenough C, Jenkins RE, Kitteringham NR, Proteomics . 2004,第10期

机译：一种从人血浆中快速消耗白蛋白和免疫球蛋白的方法
2. Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma [J] . D Johnson, D Tewksbury, J Bowen, The biochemical journal . 1976,第2期

机译：从全部人血浆中分离白蛋白和白蛋白耗尽等离子体分馏
3. Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma [J] . D Johnson, D Tewksbury, J Bowen, The biochemical journal . 1976,第2期

机译：从全部人血浆中分离白蛋白和白蛋白耗尽等离子体分馏
4. DISTRIBUTION OF PCBs IN PROTEIN DERIVATIVES (FACTOR VIII, IMMUNOGLOBULIN AND ALBUMIN) OBTAINED BY HUMAN PLASMA FRACTIONATION [C] . A. Covaci, R. Laub, M. Di Giambattista, International symposium on halogenated environmental organic pollutants and persistent organic pollutants and POPs;DIOXIN;DIOXIN 2001;POPs . 2001

机译：人血浆分离中蛋白质衍生物（因子VIII，免疫球蛋白和白蛋白）中PCBs的分布
5. Binding studies of near infrared cyanine dyes with human serum albumin and poly -L -lysine using optical spectroscopy methods. [D] . Watson, Amy Dawn. 2007

机译：使用光谱学方法研究近红外花青染料与人血清白蛋白和聚-L-赖氨酸的结合。
6. Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma. [O] . J Travis, J Bowen, D Tewksbury, 1976

机译：从整个人血浆中分离白蛋白并分离白蛋白耗尽的血浆。
7. Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma. [O] . Travis, J, Bowen, J, Tewksbury, D, 1976

机译：从整个人血浆中分离白蛋白，并分离白蛋白耗尽的血浆。
8. Intravascular Circulation and Distribution of Human 51CR-DBBF Stroma- Free Hemoglobin, 51CR-Plasma, 51-CR-Saline, 59FE-Plasma, and 125I-Albumin in the Mouse [R] . Valeri, C. R. , Ichikura, T. , Pivacek, L. E. , 1991

机译：血管内循环和分布人类51CR-DBBF基质无血红蛋白，51CR-血浆，51-CR-盐水，59FE-血浆和125I-白蛋白在小鼠中

A method for the rapid depletion of albumin and immunoglobulin from human plasma

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