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Two-dimensional maps and databases of the human macrophage proteome and secretome

机译：人巨噬细胞蛋白质组和分泌组的二维图和数据库

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摘要

Macrophages exert a crucial, but still incompletely known, role in complex disorders such inflammatory, immunological, and infectious diseases. A differential proteomic approach should help to elucidate the macrophage dysfunctions involved in these diseases. With this goal in mind, we established the first two-dimensional maps of the human macrophage proteome and secretome. Intracellular and secreted proteins were extracted from monocyte-derived macrophages obtained from healthy donors (n = 16), and separated by two-dimensional gel electrophoresis. Silver-stained gels were analyzed using Progenesis software. A high level of between-gel reproducibility was obtained, allowing us to generate two patterns specific of the macrophage proteome and secretome, respectively. A total of 127 and 66 distinct intracellular and secreted polypeptide spots, corresponding to 100 and 38 different proteins, respectively, were identified by matrix assisted laser desorption/ionisation-mass spectrometry. The two-dimensional reference maps and databases resulting from this study confirm that macrophages are involved in a wide range of biological functions, and that they provide a useful tool for a wide array of investigators involved in macrophage biology, allowing to investigate the macrophage protein changes associated with various disorders or environmental stimuli.
机译：巨噬细胞在诸如炎症，免疫和感染性疾病等复杂疾病中发挥着至关重要的作用，但仍不为人所知。差异蛋白质组学方法应有助于阐明与这些疾病有关的巨噬细胞功能障碍。考虑到这一目标，我们建立了人类巨噬细胞蛋白质组和分泌组的第一个二维图。从健康供体（n = 16）获得的单核细胞衍生的巨噬细胞中提取细胞内和分泌的蛋白，并通过二维凝胶电泳进行分离。使用Progenesis软件分析银染的凝胶。获得了高水平的凝胶间可重复性，使我们能够分别产生两种特定于巨噬细胞蛋白质组和分泌组的模式。通过基质辅助激光解吸/电离质谱法鉴定出总共127和66个不同的细胞内和分泌的多肽斑点，分别对应于100和38个不同的蛋白质。这项研究产生的二维参考图和数据库证实巨噬细胞参与了广泛的生物学功能，并且它们为参与巨噬细胞生物学的众多研究者提供了有用的工具，从而可以研究巨噬细胞蛋白质的变化与各种疾病或环境刺激有关。

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