• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proteomics >Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae)
【24h】

Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae)

机译：绿藻血球菌（绿藻科）的细胞壁蛋白质组学

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The green microalga Haematococcus pluvialis can synthesize and accumulate large amounts of the ketocarotenoid astaxanthin, and undergo profound changes in cell wall composition and architecture during the cell cycle and in response to environmental stresses. In this study, cell wall proteins (CWPs) of H. pluvialis were systematically analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) coupled with peptide mass fingerprinting (PMF) and sequence-database analysis. In total, 163 protein bands were analyzed, which resulted in positive identification of 81 protein orthologues. The highly complex and dynamic composition of CWPs is manifested by the fact that the majority of identified CWPs are differentially expressed at specific stages of the cell cycle along with a number of common wall-associated 'housekeeping' proteins. The detection of cellulose synthase orthologue in the vegetative cells suggested that the biosynthesis of cellulose occurred during primary wall formation, in contrast to earlier observations that cellulose was exclusively present in the secondary wall of the organism. A transient accumulation of a putative cytokinin oxidase at the early stage of encystment pointed to a possible role in cytokinin degradation while facilitating secondary wall formation and/or assisting in cell expansion. This work represents the first attempt to use a proteomic approach to investigate CWPs of microalgae. The reference protein map constructed and the specific protein markers obtained from this study provide a framework for future characterization of the expression and physiological functions of the proteins involved in the biogenesis and modifications in the cell wall of Haematococcus and related organisms.
机译：绿色微藻类雨生红球菌可以合成和积累大量的酮类胡萝卜素虾青素，并且在细胞周期和响应环境压力的过程中，细胞壁组成和结构会发生深刻变化。在这项研究中，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结合肽质量指纹分析（PMF）和序列数据库分析，系统地分析了幽门螺杆菌的细胞壁蛋白（CWPs）。总共分析了163个蛋白条带，从而鉴定出81个蛋白直向同源物。 CWP的高度复杂和动态组成体现在以下事实：大多数已识别的CWP与许多常见的与壁相关的“管家”蛋白在细胞周期的特定阶段差异表达。营养细胞中纤维素合酶直向同源物的检测表明，纤维素的生物合成发生在初生壁形成过程中，这与早期观察到的纤维素仅存在于生物体次生壁中相反。包囊早期阶段假定的细胞分裂素氧化酶的短暂积累表明在细胞分裂素降解中可能发挥作用，同时促进继发壁形成和/或协助细胞扩增。这项工作代表了使用蛋白质组学方法研究微藻CWP的首次尝试。从本研究中构建的参考蛋白质图谱和获得的特定蛋白质标志物为以后表征血球菌和相关生物的生物发生和修饰的蛋白质的表达和生理功能提供了框架。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号