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首页> 外文期刊>Proteomics >Molecular interactions between wheat and cereal aphid (Sitobion avenae): analysis of changes to the wheat proteome.
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Molecular interactions between wheat and cereal aphid (Sitobion avenae): analysis of changes to the wheat proteome.

机译：小麦和谷物蚜虫（Sitobion avenae）之间的分子相互作用：分析小麦蛋白质组的变化。

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摘要

Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat seedlings after extraction and 2-DE. Sixty-seven spots differed significantly between control and infested plants following 24 h of aphid feeding, with 27 and 11 up-regulated, and 8 and 21 down-regulated, in local or systemic tissues, respectively. After 8 days, 80 protein spots differed significantly between control and aphid treatments with 13 and 18 up-regulated and 27 and 22 down-regulated in local or systemic tissues, respectively. As positive controls, plants were treated with salicylic acid or methyl jasmonate; 81 and 37 differentially expressed protein spots, respectively, were identified for these treatments. Approximately, 50% of differentially expressed protein spots were identified by PMF, revealing that the majority of proteins altered by aphid infestation were involved in metabolic processes and photosynthesis. Other proteins identified were involved in signal transduction, stress and defence, antioxidant activity, regulatory processes, and hormone responses. Responses to aphid attack at the proteome level were broadly similar to basal non-specific defence and stress responses in wheat, with evidence of down-regulation of insect-specific defence mechanisms, in agreement with the observed lack of aphid resistance in commercial wheat lines.
机译：蚜虫是谷类作物的主要害虫，既是病毒的载体，又会造成直接危害。在蛋白质组学分析中研究了小麦对谷物蚜虫（Sitobion avenae）侵染的反应。提取和2-DE后，在小麦幼苗叶片提取物中可重复检测到约500个蛋白斑点。蚜虫取食24 h后，对照和受侵染植物之间有67个斑点显着不同，分别在局部或全身组织中有27和11个上调，而8和21个下调。 8天后，对照和蚜虫处理之间的80个蛋白点有显着差异，局部或全身组织中分别有13和18个上调，而27和22个下调。作为阳性对照，用水杨酸或茉莉酸甲酯处理植物;对于这些处理，分别鉴定出81个和37个差异表达的蛋白质斑点。大约有50％的差异表达蛋白斑点被PMF鉴定，表明蚜虫侵染改变的大多数蛋白都参与了代谢过程和光合作用。鉴定出的其他蛋白质与信号转导，压力和防御，抗氧化活性，调节过程和激素反应有关。在蛋白质组水平上对蚜虫侵袭的反应与小麦的基础非特异性防御​​和胁迫反应大致相似，有昆虫特异性防御​​机制下调的证据，这与在商业小麦品系中观察到的蚜虫抗性缺乏一致。

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