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首页> 外文期刊>Propagation of Ornamental Plants >Micropropagation of Rhododendron keiskei var. hypoglaucum Suto & Suzuki and assessment of clonal fidelity of plantlets by RAPD.
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Micropropagation of Rhododendron keiskei var. hypoglaucum Suto & Suzuki and assessment of clonal fidelity of plantlets by RAPD.

机译：杜鹃杜鹃的微繁殖。 Sug＆Suzuki的hypoglaucum以及通过RAPD评估幼苗的克隆保真度。

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摘要

An efficient reproducible protocol has been developed for in vitro propagation of Rhododendron keiskei var. hypoglaucum. Nodal explants were cultured on Anderson basal nutrients (AM) medium supplemented with different concentrations and combinations of plant growth regulators for axillary shoot proliferation. The highest percentage of shoot induction (96%) was achieved when nodal explants were cultured on AM medium supplemented with 2.0 mg l-1 2iP, 0.5 mg l-1 IAA, and 1.0 mg l-1 GA3 with a mean of 16 shoots per explant. Individual shoots, which were grown to about 2-3 cm long, were transferred onto a full or half strength AM medium with 0, 0.5, 1.0, 2.0 or 4.0 mg l-1 IBA or NAA for rooting. The highest percentage of rooting (100%), with maximum number of 7.6 roots per shoot and the greatest root length (4.4 cm) were obtained on the half-strength AM medium supplemented with 0.5 mg l-1 IBA. Random amplified polymorphic DNA analysis confirmed that the regenerated plantlets were genetically identical to their donor plant.
机译：已经开发出一种有效的可重复方案，用于杜鹃花杜鹃的体外繁殖。胚珠。节点外植体在添加了不同浓度的安德森基础营养素（AM）培养基和植物生长调节剂组合中培养，以促进腋芽生长。当在添加了2.0 mg l -1 2iP，0.5 mg l -1 IAA，和1.0 mg l -1 GA 3 ，平均每个外植体芽16个。将长至2-3 cm长的单个芽转移到具有0、0.5、1.0、2.0或4.0 mg l -1 IBA或NAA的完全或半强度AM培养基上生根。在补充0.5 mg l -1 的半强度AM培养基上获得最高的生根率（100％），每枝最大生根数7.6，最大根长（4.4 cm）。 IBA。随机扩增的多态性DNA分析证实，再生的小苗在遗传上与其供体植物相同。

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