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首页> 外文期刊>Protein Expression and Purification >Purification of native dehydrin from Glycine Max cv., Pisum sativum, and Rosmarinum officinalis by affinity chromatography
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Purification of native dehydrin from Glycine Max cv., Pisum sativum, and Rosmarinum officinalis by affinity chromatography

机译：通过亲和色谱法从大豆，豌豆和迷迭香中纯化天然脱水素

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摘要

An improved method for the purification of dehydrin from soy (glycine max) is described. Acidic extraction of soy whey was followed by a three step chromatographic process: capture on copper charged Chelating Sepharose Big Beads, intermediate hydrophobic interaction chromatography on Source 15 PHE, and a polishing step on blue Sepharose. The 32-kDa native soy dehydrin was purified to a purity of greater than 98.5% with an overall recovery of 63%. When compared to a previously published purification procedure, recovery, time requirements, and sample preparation steps were improved. The developed method is readily scaleable. Preliminary results show that the process can be used for dehydrins from rosemary (Rosmarinum officinalis) and pea (Pisum sativum). (C) 2002 Elsevier Science (USA). All rights reserved. [References: 41]
机译：描述了一种从大豆中纯化脱水素的改进方法（最大甘氨酸）。大豆乳清的酸性萃取之后是三步色谱过程：在带铜的螯合琼脂糖凝胶大珠子上捕获，在Source 15 PHE上进行中间疏水相互作用色谱，在蓝色琼脂糖凝胶上进行抛光。将32 kDa天然大豆脱水素纯化至大于98.5％的纯度，总回收率为63％。与以前发布的纯化程序相比，回收率，时间要求和样品制备步骤得到了改善。所开发的方法易于扩展。初步结果表明该方法可用于迷迭香（Rosmarinum officinalis）和豌豆（Pisum sativum）中的脱水素。 （C）2002 Elsevier Science（美国）。版权所有。 [参考：41]

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