Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli

Liu YF.; Ma RL.; Wang SL.; Duan ZY.; Zhang JH.; Wu LJ.; Wu CF.

首页> 外文期刊>Protein Expression and Purification >Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli

【24h】

Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli

机译：中国蝎子Buthus martensii karsch毒液中抗肿瘤止痛肽在大肠杆菌中的表达

获取原文

获取原文并翻译 | 示例

获取外文期刊封面封底 >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The gene encoding a putative mature antitumor-analgesic peptide (AGAP) from the venom of the Chinese scorpion Buthus martensii Karsch was obtained by polymerase chain reaction (PCR) according to its cDNA sequence and expressed in Escherichia coli. While most of the recombinant AGAP was expressed in the form of insoluble inclusion body. The recombinant AGAP was purified to homogeneity by metal chelating affinity chromatography. Pharmaceutical tests showed that the recombinant AGAP has both analgesic and antitumor activities on mice. (C) 2002 Elsevier Science (USA). All rights reserved. [References: 34]

机译：通过聚合酶链反应（PCR），从中国蝎子Buthus martensii Karsch的毒液中获得了一种推定的成熟抗肿瘤止痛肽（AGAP）编码基因，并在大肠杆菌中表达。而大多数重组AGAP以不溶性包涵体的形式表达。通过金属螯合亲和层析将重组的AGAP纯化至均质。药物测试表明，重组AGAP对小鼠具有止痛和抗肿瘤活性。（C）2002 Elsevier Science（美国）。版权所有。 [参考：34]

著录项

来源
《Protein Expression and Purification》 |2003年第2期|共6页
作者
Liu YF.; Ma RL.; Wang SL.; Duan ZY.; Zhang JH.; Wu LJ.; Wu CF.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类蛋白质;
关键词
Depressant insect toxin; Anti-epilepsy peptide; Molecular characterization; K+ channels; Bmk m1; Neurotoxin; Purification; Crystallization; Sequence; Cdna;

机译：抑制性昆虫毒素;抗癫痫肽;分子表征;K +通道;Bmk m1;神经毒素;纯化;结晶;序列;Cdna;

相似文献

外文文献
中文文献
专利

1. Extract from Buthus martensii Karsch is associated with potassium channels on glioma cells [J] . Mingxian Li, Hongmei Meng, Shao Wang, 中国神经再生研究（英文版） . 2011,第015期
2. Effects of antigliomatin from the scorpion venom of Buthus martensii Karsch on chloride channels on C6 glioma cells [J] . Zan Wang, Mingxian Li, Hongmei Meng, 中国神经再生研究（英文版） . 2011,第018期
3. Effects of antigliomatin from the scorpion venom of Buthus martensii Karsch on chloride channels on C6 glioma cells [J] . Zan Wang, Mingxian Li, Hongmei Meng, 中国神经再生研究：英文版 . 2011,第018期
4. Polyclonal antibody against an insect excitatory toxin BmKIT from Buthus Martensii karsch and detection of BmKIT expressed in transgenic cotton [J] . Hao Chanjuan, Xu Chenggang, Zhang Zhiyun, 高技术通讯（英文版） . 2008,第002期
5. Effect of scorpion venom analgesic active peptide extracted from Buthus martensii Karsch on evoked potential in the thalamic posterior nucleus group in rats [J] . Qiuhong Lin, Xinxin Li 中国神经再生研究（英文版） . 2008,第004期
6. Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli [J] . Liu YF., Ma RL., Wang SL., Protein Expression and Purification . 2003,第2期

机译：中国蝎子Buthus martensii karsch毒液中抗肿瘤止痛肽在大肠杆菌中的表达
7. Expression and Purification of an Antitumor-Analgesic Peptide from the Venom of Mesobuthus martensii Karsch by Small Ubiquitin-Related Modifier Fusion in Escherichia coli [J] . Peng Cao, Jiemiao Yu, Wuguang Lu Biotechnology Progress . 2010,第5期

机译：小泛素相关修饰剂融合在大肠杆菌中表达和纯化马氏肉Me毒液中的抗肿瘤镇痛肽
8. Expression of Anti-Neuroexcitation Peptide (ANEP) of Scorpion Buthus Martensii Karsch In Escherichia Coli [J] . Jing-Hai Zhang, Zi-Chun Hua, Zhao Xu, Preparative biochemistry & biotechnology: An international journal for rapid communication . 2001,第1期

机译：蝎子马氏杆菌Karsch的抗神经兴奋肽（ANEP）在大肠杆菌中的表达
9. Effects of Buthus Martensii Karsch Venom on Cell Proliferation and Cytotoxicity in Hela Cells [C] . Wang Zhi, Fu Wen-hui, Lu Xiang-yang, International Conference on Smart Materials and Nanotechnology in Engineering . 2012

机译：Buthus Martensii Karsch Veom对HeLa细胞细胞增殖和细胞毒性的影响
10. Exploring the conditions for the expression of low molecular weight glutenin 1D1 protein in Escherichia coli (E. coli). [D] . Gudiseva, Venkata Harini. 2006

机译：探索低分子量谷蛋白1D1蛋白在大肠杆菌（E. coli）中表达的条件。
11. Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch. [O] . X H Zhou, D Yang, J H Zhang, 1989

机译：来自蝎子Buthus martensii Karsch毒液的抗癫痫肽的纯化和N末端部分序列。
12. Molecular cloning and sequencing of two ‘short chain’ and two ‘long chain’ K+ channel-blocking peptides from the Chinese scorpion Buthus martensii Karsch [O] . Zhu Shunyi, Li Wenxin, Zeng Xianchun, 1999

机译：来自中国蝎子Buthus martensii Karsch的两个“短链”和两个“长链” K +通道封闭肽的分子克隆和测序

Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli

摘要

著录项

相似文献

相关主题

期刊订阅