An E. coli expression system optimized for DELLA proteins

Sun XL; Frearson N; Kirk C; Jones WT; Harvey D; Rakonjac J; Foster T; Al-Samarrai T

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An E. coli expression system optimized for DELLA proteins

机译：针对DELLA蛋白优化的大肠杆菌表达系统

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The DELLA proteins are involved in regulation of plant growth in response to phytohormonal signals such as GA, ethylene, and auxin. They have become one of most challenging and active area of research due to their fundamental roles in plant biology. Here, we describe the first successful expression of the N-terminal domains of DELLA proteins of Arabidopsis thaliana and Malus domestica in Escherichia coli system which will be used to produce monoclonal antibodies for profiling protein micro-arrays. Optimizations of the cloning, expression, and purification using specific tags have been discussed. (c) 2007 Elsevier Inc. All rights reserved.

机译：DELLA蛋白参与响应植物激素信号（例如GA，乙烯和生长素）的植物生长调节。由于它们在植物生物学中的基本作用，它们已成为最具挑战性和最活跃的研究领域之一。在此，我们描述了拟南芥和家蝇的DELLA蛋白N末端结构域在大肠杆菌系统中的首次成功表达，该系统将用于生产用于分析蛋白质微阵列的单克隆抗体。已经讨论了使用特定标签优化克隆，表达和纯化的方法。（c）2007 Elsevier Inc.保留所有权利。

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《Protein Expression and Purification》 |2008年第1期|共7页
作者
Sun XL; Frearson N; Kirk C; Jones WT; Harvey D; Rakonjac J; Foster T; Al-Samarrai T;
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正文语种 eng
中图分类蛋白质;
关键词
DELLA proteins; E. coli expression; gibberellin signal transduction pathway; plant proteins; ESCHERICHIA-COLI; PROTEOLYTIC DEGRADATION; FUSION PROTEIN; GIBBERELLIN; GROWTH; PURIFICATION; REPRESSOR; ANTIBODY; ENCODES; BINDING;

机译：DELLA蛋白;E.大肠杆菌表达;赤霉素信号传导途径;植物蛋白;大肠埃希菌;蛋白水解;融合蛋白;赤霉素;生长;纯化;阻遏物;抗体;编码;结合;

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1. An E. coli expression system optimized for DELLA proteins [J] . Sun XL, Frearson N, Kirk C, Protein Expression and Purification . 2008,第1期

机译：针对DELLA蛋白优化的大肠杆菌表达系统
2. Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system [J] . Xinying Shi, Ti Wu, Christian M. Cole, Scientific reports. . 2018,第1期

机译：基于大肠杆菌提取物的无细胞表达系统中ClpXP活性和蛋白质合成的优化
3. Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems [J] . Zelasko S., Palaria A., Das A. Protein Expression and Purification . 2013,第1期

机译：使用大肠杆菌表达系统进行优化以实现细胞色素P450蛋白的高水平表达
4. CLONING, EXPRESSION AND BIOACTIVITY OF HEMAGGLUTININBINDING DOMAIN PROTEIN OF AVIAN INFLUENZA VIRUSIN THE YEAST PICHIA PASTORIS AND E. COLI [C] . Zhou Kai, He Hongxuan, Wang Xinwei, The 6'th International Forum on Post-genome Technologies(6'IFPT)（第六届国际后基因组生命科学技术学术论坛） . 2009

机译：酵母毕赤酵母和大肠杆菌禽流感病毒结合血凝素的域蛋白的克隆，表达和生物活性。
5. Subcloning, Expression and Purification of Functional E. coli Nucleotide Excision Repair Protein UvrA Using IMPACT-CN System. [D] . Lin, Cathy W. 2014

机译：使用IMPACT-CN系统进行功能性大肠杆菌核苷酸切除修复蛋白UvrA的亚克隆，表达和纯化。
6. Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study [O] . Weiqing Zhang, Jing Lu, Shuwen Zhang, 2018

机译：通过比较和优化信号肽开发一种在大肠杆菌中表达重组蛋白的有效系统：以荧光假单胞菌BJ-10热稳定脂肪酶的表达为例
7. Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system [O] . Xinying Shi, Ti Wu, Christian M. Cole, 2018

机译：基于大肠杆菌提取物的无细胞的无细胞表达系统中CLPXP活性和蛋白质合成的优化

An E. coli expression system optimized for DELLA proteins

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