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首页> 外文期刊>Protein Expression and Purification >Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells
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Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells

机译：具有人工开放阅读框的优选人密码子优化的高斯荧光素酶基因在哺乳动物和细菌细胞中的蛋白表达

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摘要

The protein expressions of three preferred human codon-optimized Gaussia luciferase genes (pGLuc, EpGLuc, and KpGLuc) were characterized in mammalian and bacterial cells by comparing them with those of wild-type Gaussia luciferase gene (wGLuc) and human codon-optimized Gaussia luciferase gene (hGLuc). Two synthetic genes of EpGLuc and KpGLuc containing the complete preferred human codons have an artificial open-reading frame; however, they had the similar protein expression levels to those of pGLuc and hGLuc in mammalian cells. In bacterial cells, the protein expressions of pGLuc, EpGLuc, and KpGLuc with approximately 65% GC content were the same and showed approximately 60% activities of wGLuc and hGLuc. The artificial open-reading frame in EpGLuc and KpGLuc did not affect the protein expression in mammalian and bacterial cells. (C) 2016 Elsevier Inc. All rights reserved.
机译：通过比较哺乳动物和细菌细胞与野生型高斯荧光素酶基因（wGLuc）和人密码子优化的高斯荧光素酶的特征，鉴定了三种优选的人密码子优化的高斯荧光素酶基因的蛋白表达（pGLuc，EpGLuc和KpGLuc）。基因（hGLuc）。包含完整的首选人密码子的EpGLuc和KpGLuc的两个合成基因具有人工开放阅读框。然而，它们在哺乳动物细胞中的蛋白表达水平与pGLuc和hGLuc相似。在细菌细胞中，GC含量约为65％的pGLuc，EpGLuc和KpGLuc的蛋白质表达相同，并显示wGLuc和hGLuc的活性约为60％。 EpGLuc和KpGLuc中的人工开放阅读框不影响哺乳动物和细菌细胞中的蛋白质表达。 （C）2016 Elsevier Inc.保留所有权利。

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