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首页> 外文期刊>Protein Expression and Purification >High yield expression, refolding, and characterization of recombinant interferon alpha 2/alpha 8 hybrids in Escherichia coli
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High yield expression, refolding, and characterization of recombinant interferon alpha 2/alpha 8 hybrids in Escherichia coli

机译：重组干扰素α2 /α8杂种在大肠杆菌中的高产量表达，重折叠和表征

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摘要

Interferons (IFNs) are a family of pleiotropic cytokines used for the treatment of various viral infections and cancers. The low-cost production of IFNs with high biological value and the discovery of IFNs with improved properties are important for the treatment of these diseases as well as for understanding the physiological functions of these compounds. We describe a protein expression system for the production of IFNs alpha2, alpha8, and their hybrids in insoluble form in Escherichia coli, coupled to an efficient two-step optimized refolding and histidine-tag purification protocol. The expressed IFNs were of high biological value, as shown in antiviral and antiproliferative assays and some had specific activities higher than those of the commercially available interferon preparations and exhibited novel properties. This time-efficient, optimized protein expression method allows for the production of not just a single interferon subtype but several native and hybrid IFNs with relatively high yield and low cost that can be used in functional and potentially clinical assays. (C) 2003 Elsevier Science (USA). All rights reserved. [References: 36]
机译：干扰素（IFN）是用于治疗各种病毒感染和癌症的多效性细胞因子家族。低成本生产具有高生物学价值的IFN和发现具有改善特性的IFN对这些疾病的治疗以及理解这些化合物的生理功能很重要。我们描述了一种蛋白质表达系统，用于在大肠杆菌中以不溶性形式生产IFNsα2，α8及其杂合体，并结合了有效的两步优化重折叠和组氨酸标签纯化方案。如抗病毒和抗增殖试验所示，表达的IFN具有很高的生物学价值，并且某些具有比市售干扰素制剂更高的比活性，并表现出新颖的特性。这种省时，优化的蛋白质表达方法不仅可以生产单个干扰素亚型，还可以以相对较高的产量和低成本生产多种天然干扰素和杂合干扰素，可用于功能性和潜在的临床检测。 （C）2003 Elsevier Science（美国）。版权所有。 [参考：36]

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