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首页> 外文期刊>Protein Expression and Purification >The purification of IgY from chicken egg yolk by preparative electrophoresis
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The purification of IgY from chicken egg yolk by preparative electrophoresis

机译：制备型电泳从蛋黄中纯化IgY

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Chicken IgY has been purified from egg yolk by preparative electrophoresis on the Gradiflow, a system which has been employed for the purification of a wide range of proteins with high recovery and biological activity. Protein purification on the Gradiflow utilises electrophoresis with selected combinations of porous membranes and buffers. The purification of IgY was achieved by initial PEG lipid precipitation, then a single step Gradiflow run by a strategy based on the relatively high pI range of IgY compared to other egg yolk proteins. The IgY yields obtained from the delipidised supernatant are consistently greater than 80% by immunoassay. The purity of the IgY fraction compared favourably with IgY prepared using three commercial products. (C) 2003 Elsevier Science (USA). All rights reserved. [References: 23]
机译：鸡IgY已通过在Gradiflow上进行制备性电泳从蛋黄中纯化出来，该系统已用于纯化具有高回收率和生物活性的多种蛋白质。 Gradiflow上的蛋白质纯化利用电泳以及多孔膜和缓冲液的选定组合。 IgY的纯化是通过最初的PEG脂质沉淀实现的，然后通过基于与其他蛋黄蛋白相比较高的IgY的pI范围的策略进行一步Gradiflow。通过免疫测定，从脱脂的上清液获得的IgY产率始终大于80％。与使用三种市售产品制备的IgY相比，IgY级分的纯度令人满意。 （C）2003 Elsevier Science（美国）。版权所有。 [参考：23]

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