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首页> 外文期刊>Psychopharmacology >Chronic prevention of micro-opioid receptor (MOR) G-protein coupling in the pontine parabrachial nucleus persistently decreases consumption of standard but not palatable food.
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Chronic prevention of micro-opioid receptor (MOR) G-protein coupling in the pontine parabrachial nucleus persistently decreases consumption of standard but not palatable food.

机译：长期预防桥臂臂旁核中的微阿片受体（MOR）G蛋白偶联持续减少标准食物的摄入量，但不能减少可口食物的摄入量。

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RATIONALE: Acute pharmacological studies implicate mu-opioid receptors (MORs) in the parabrachial nucleus (PBN) of the brainstem in modulating eating. The long-term effects of preventing the cellular function of parabrachial MORs on food consumption remain to be elucidated. OBJECTIVES: To determine whether (1) chronic inhibition of MOR-mediated G-protein coupling in the PBN of rats would persistently reduce eating and (2) food properties dictate the effects of MOR blockade. MATERIALS AND METHODS: We microinfused the irreversible MOR antagonist, beta-funaltrexamine (beta-FNA) into the lateral PBN and measured the intake of standard and calorically dense palatable chow for 1 week. First, rats were given standard chow for 20 h daily and a calorically dense palatable chow for 4 h during the day. We infused the agonist, [D: -Ala(2), N-Me-Phe(4), Glycinol(5)]-Enkephalin (DAMGO), 1 week after beta-FNA to probe the acute effects of exogenous stimulation of MORs on palatable food intake. [(35)S]GTPgammaS autoradiography quantified regional loss of MOR cellular function. Next, we measured the actions of beta-FNA on food intake in rats given only standard or palatable chow for 1 week. RESULTS: One infusion of beta-FNA persistently decreased consumption of standard but not palatable chow, regardless of feeding regimen. beta-FNA also blocked DAMGO-stimulated palatable chow intake, prevented DAMGO-stimulated G-protein coupling in the central and external lateral subnuclei of the PBN, and decreased coupling in the medial PBN. beta-FNA did not affect kappa-opioid receptors. CONCLUSIONS: MORs in the lateral PBN serve a physiological role in stimulating consumption of standard food. Properties of the diet, such as high palatability or caloric density, may override the influence of inhibiting MOR function.
机译：理由：急性药理研究表明，在调节饮食过程中，脑干的臂旁臂核（PBN）中存在阿片受体（MOR）。防止臂旁MORs的细胞功能对食物消耗的长期影响仍有待阐明。目的：确定（1）慢性抑制大鼠PBN中MOR介导的G蛋白偶联是否会持续减少进食和（2）食物特性决定MOR阻断的作用。材料与方法：我们将不可逆的MOR拮抗剂β-富纳曲胺（β-FNA）微注入了PBN外侧，并测量了标准和热量密集的可口食物的摄入量，为期1周。首先，给大鼠每天20小时的标准食物，一天中4小时的卡路里密集的可口食物。我们在β-FNA后1周注入了激动剂[D：-Ala（2），N-Me-Phe（4），甘醇（5）]-脑啡肽（DAMGO），以研究外源性刺激对MORs的急性影响摄入适量的食物。 [（35）S] GTPgammaS放射自显影术定量了MOR细胞功能的区域性丧失。接下来，我们测量了仅给予标准或可口食物1周的大鼠中β-FNA对食物摄入的作用。结果：无论喂养方式如何，一次输注β-FNA都会持续减少标准食物的摄入量，但不会减少可口的食物。 β-FNA还可阻断DAMGO刺激的可口食物的摄入，防止PBN的中央和外部外侧亚核中DAMGO刺激的G蛋白偶联，并减少PBN内侧的偶联。 β-FNA不会影响κ阿片受体。结论：外侧PBN中的MOR在刺激标准食品的消费中起着生理作用。饮食的特性，例如高适口性或卡路里密度，可能会超过抑制MOR功能的影响。

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