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首页> 外文期刊>Postharvest Biology and Technology >Expression of a lipoxygenase encoding gene (BoLOX1) during postharvest senescence of broccoli.
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Expression of a lipoxygenase encoding gene (BoLOX1) during postharvest senescence of broccoli.

机译：花椰菜采后衰老过程中脂氧合酶编码基因（ BoLOX1 ）的表达。

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摘要

Lipoxygenases (LOX) belong to a large family of plant enzymes that catalyze the hydroperoxidation of polyunsaturated fatty acids. Most of them are expressed during senescence and contribute to membrane deterioration and biosynthesis of jasmonic acid, a known senescence enhancer. In this work, we cloned a fragment of a gene encoding a LOX from broccoli (BoLOX1). The analysis of the sequence revealed that BoLOX1 is closely related to other LOX from higher plants. Furthermore, we analyzed the expression of BoLOX1 and detected a larger increase during postharvest senescence. A slight increase of total lipoxygenase activity was also found during senescence. In other sets of experiments, broccoli heads were treated with plant hormones, such as cytokinin and ethylene, as a way to assess the effect of such compounds on the expression of BoLOX1. Cytokinin treatment delayed the increase of BoLOX1 expression and lipoxygenase activity whereas ethylene accelerated both processes. Also, several postharvest treatments were applied in order to delay senescence in broccoli florets and to evaluate their effects on BoLOX1 expression. Samples treated with modified atmosphere, hot air, UV-C or white light showed a delay in chlorophyll degradation and degreening. In most cases, the treatments also delayed the increase of BoLOX1 expression, reaffirming the relationship between the expression of this gene and senescence. However, treatments like modified atmospheres and visible light markedly increased lipoxygenase activity, which suggests a lack of correlation between BoLOX1 expression and lipoxygenase activity
机译：脂氧合酶（LOX）属于一大类植物酶，可催化多不饱和脂肪酸的加氢过氧化反应。它们中的大多数在衰老过程中表达，并导致茉莉酸（一种已知的衰老促进剂）的膜变质和生物合成。在这项工作中，我们从西兰花（ BoLOX1 ）克隆了一个编码LOX的基因的片段。序列分析表明， BoLOX1 与高等植物的其他LOX密切相关。此外，我们分析了 BoLOX1 的表达，并发现了采后衰老过程中更大的增加。在衰老过程中还发现总脂氧合酶活性略有增加。在其他实验组中，用植物激素（例如细胞分裂素和乙烯）处理西兰花头部，以评估此类化合物对 BoLOX1 表达的影响。细胞分裂素处理延迟了BoLOX1表达和脂氧合酶活性的增加，而乙烯则加速了这两个过程。另外，为了延迟西兰花小花的衰老并评估其对 BoLOX1 表达的影响，还采用了几种收获后处理方法。用改良的气氛，热空气，UV-C或白光处理的样品显示出叶绿素降解和减缓的延迟。在大多数情况下，这些治疗方法还延迟了 BoLOX1 表达的增加，从而重申了该基因的表达与衰老之间的关系。然而，诸如改变气氛和可见光之类的处理显着增加了脂氧合酶活性，这表明 BoLOX1 表达与脂氧合酶活性之间缺乏相关性。

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