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首页> 外文期刊>Postharvest Biology and Technology >Increase in DNA fragmentation and the role of ethylene and reactive oxygen species in petal senescence of Osmanthus fragrans
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Increase in DNA fragmentation and the role of ethylene and reactive oxygen species in petal senescence of Osmanthus fragrans

机译：桂花花瓣衰老过程中DNA片段的增加以及乙烯和活性氧的作用

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In sweet osmanthus (Osmanthus fragrans Lour.) flowers, petal browning, partial abscission and wilting are the visible symptoms of senescence, 4-5 days after flowering, which seriously affect its ornamental and economic value. In the present study, DNA fragmentation was used as a marker to investigate the role of ethylene and reactive oxygen species (ROS) in flower senescence of O. fragrans 'Liuye Jingui'. In intact plants, nuclear shrinkage and DNA fragmentation occurred at the late full flowering stage when senescence symptoms became visible. This coincided with a rapid increase in ethylene production, ROS generation and lipid peroxidation. To further determine the role of ethylene and ROS in flower senescence, cut flowers were treated with ethephon, silver thiosulphate (STS), hydrogen peroxide (H2O2), and vitamin C (Vc). The vase life of cut flowers was significantly prolonged by 0.2 mM STS and 2.5 mM Vc treatments, but reduced by 0.03% H2O2 and 500 mg L-1 ethephon treatments, compared to distilled water. The percentage of DNA fragmentation was dramatically increased by ethephon but reduced by STS treatment throughout vase life. In contrast, the dramatic increase of DNA fragmentation in the H2O2 treated samples was only observed at day three, and clear petal abscission and rapid petal wilting occurred only with ethephon. Compared with the distinguishable nuclei and complete vacuoles in both STS treatment and distilled water, ethephon treatment caused substantial damage to large central vacuoles and other organelles, and many petal cells twisted out of shape due to a loss of cytoplasm, resulting in rapid petal wilting. Thus, it is concluded that ethylene plays an important role in flower senescence of sweet osmanthus, enhancing DNA fragmentation, damaging cellular structure, and leading to petal abscission and wilting. In addition, ROS is also involved in the regulation of late DNA degradation and lipid peroxidation
机译：在甜桂花（Osmanthus fragrans Lour。）花中，开花后4-5天可见的花瓣褐变，部分脱落和枯萎是衰老的可见症状，严重影响了其观赏和经济价值。在本研究中，DNA片段化被用作标记，以研究乙烯和活性氧（ROS）在东方草莓“六叶金桂”的花衰老中的作用。在完整的植物中，当衰老症状变得明显时，核收缩和DNA断裂发生在完整的开花后期。这与乙烯产量，ROS生成和脂质过氧化的快速增加相吻合。为了进一步确定乙烯和ROS在花衰老中的作用，用乙烯利，硫代硫酸银（STS），过氧化氢（H2O2）和维生素C（Vc）处理切花。与蒸馏水相比，切花的花瓶寿命显着延长了0.2 mM STS和2.5 mM Vc处理，但减少了0.03％H2O2和500 mg L-1乙烯利处理。乙烯利显着增加了DNA片段化的百分比，但在整个花瓶寿命中，通过STS处理降低了DNA片段化的百分比。相反，仅在第三天观察到过H2O2处理的样品中DNA片段的急剧增加，只有乙烯利才出现清晰的花瓣脱落和快速的枯萎现象。与STS处理和蒸馏水中可分辨的核和完全液泡相比，乙烯利处理对大型中央液泡和其他细胞器造成了实质性损害，许多花瓣细胞由于细胞质损失而扭曲变形，导致花瓣迅速萎。因此，可以得出结论，乙烯在桂花的花衰老中起着重要作用，增加了DNA片段化，破坏了细胞结构，并导致花瓣脱落和枯萎。此外，ROS还参与后期DNA降解和脂质过氧化的调节

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