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首页> 外文期刊>Potato Journal >IN-VITRO DETACHED LEAF ASSAY OF HOST-MEDIATED RNAi LINES CARRYING PHYTOPHTHORA INFESTANS AVR3A EFFECTOR GENE FOR LATE BLIGHT RESISTANCE
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IN-VITRO DETACHED LEAF ASSAY OF HOST-MEDIATED RNAi LINES CARRYING PHYTOPHTHORA INFESTANS AVR3A EFFECTOR GENE FOR LATE BLIGHT RESISTANCE

机译：携带致病疫霉菌AVR3A效应基因的寄主植物介导的RNAi系的离体叶片分析

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Phytophthora infestans, the causal organism of potato late blight adversely affects the quality as well as quantity of the potato crop. Late blight is the most dreaded biotic stress of potato till date. Although, a number of management strategies havebeen developed for the effective management of the disease but the fast evolving virulent strains of P. infestans still pose a challenge. The RXLR effector gene Avr3a from P. infestans has been silenced through small interfering RNA (siRNA) and artificial micro RNA (amiRNA) techniques, as Avr3a has been demonstrated to be essential factor for virulence in P. infestans. Phenotyping of Kufri Khyati and Kufri Pukhraj lines of potato developed through siRNA and amiRNA for late blight resistance was undertaken using detached leaf assay. The siRNA derived lines Kufri Khyati 1037 and Kufri Khyati 1129 and amiRNA derived line Kufri Khyati 2.1358 exhibited less lesion area as well as spore production and categorized as moderately resistant. Also, the reduced expression of Avr3a transcript in the siRNA derived lines Kufri Khyati 1037 and Kufri Khyati 1129 and amiRNA derived line Kufri Khyati 2.1358 coincided with the less disease severity in the leaves of transformed plants. Although both techniques of RNAi had remarkable effect on the pathogenicity of the gene Avr3a still siRNA mediated technique of silencing performed better than amiRNA mediated gene silencing when tested through detached leaf assay.
机译：马铃薯晚疫病的致病菌疫霉（Phytophthora infestans）对马铃薯作物的质量和数量产生不利影响。迄今为止，晚疫病是马铃薯最可怕的生物胁迫。尽管已经开发出许多用于有效控制该疾病的管理策略，但是快速发展的致病性致病力致病菌株仍然构成挑战。由于已经证明了Avr3a是致病性致病力的重要因素，因此已通过小干扰RNA（siRNA）和人工微RNA（amiRNA）技术使来自致病性致病菌的RXLR效应基因Avr3a沉默。使用离体叶片试验对通过siRNA和amiRNA开发的马铃薯的Kufri Khyati和Kufri Pukhraj品系进行了表型分型，以用于后期抗白叶枯病。 siRNA衍生品系Kufri Khyati 1037和Kufri Khyati 1129以及amiRNA衍生品系Kufri Khyati 2.1358表现出较小的病灶面积和孢子产生，并被归类为中度耐药。同样，Avr3a转录物在siRNA衍生品系Kufri Khyati 1037和Kufri Khyati 1129和amiRNA品系Kufri Khyati 2.1358中的表达降低，与转化植物叶片中病害严重程度较小相吻合。尽管两种RNAi技术均对Avr3a基因的致病性具有显着影响，但是当通过离体叶片测定法进行测试时，siRNA介导的沉默技术的效果要好于amiRNA介导的基因沉默。

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