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首页> 外文期刊>Chemistry: A European journal >Ionic Liquids as Selectors for the Enhanced Detection of Proteins
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Ionic Liquids as Selectors for the Enhanced Detection of Proteins

机译：离子液体作为增强蛋白质检测的选择剂

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摘要

Herein, we report an approach for protein detection enhanced by ionic liquid (IL) selectors in capillary electrophoresis (CE), with avidin as a model protein. Hydrophilic ILs were added into the running buffer of CE and acted as selectors for sample injection, enriching the positive target and excluding the negative from the capillary. When using 3% (v/v) IL selector, the detection sensitivity of avidin was improved by over one order of magnitude, while the interference from pro-tein adsorption was effectively avoided, even in an uncoated capillary. The elec-trochemiluminescence method was initially used for IL-based CE with low noise that was independent of the IL concentration, making ILs almost transparent as additives in the electrophoresis buffer.
机译：在本文中，我们报告了一种通过亲和素作为模型蛋白的毛细管电泳（CE）中的离子液体（IL）选择器增强的蛋白质检测方法。将亲水性IL添加到CE的运行缓冲液中，并充当样品进样的选择器，从而富集阳性靶标并从毛细管中排除阴性靶标。当使用3％（v / v）IL选择剂时，抗生物素蛋白的检测灵敏度提高了一个数量级以上，而即使在未涂层的毛细管中，也能有效避免蛋白质吸附的干扰。电化学发光法最初用于具有低噪声的基于IL的CE，该方法与IL浓度无关，从而使IL在电泳缓冲液中作为添加剂几乎透明。

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