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An unexpected new optimum in the structure space of DNA solubilizing single-walled carbon nanotubes

机译:DNA增溶单壁碳纳米管的结构空间中出乎意料的新优化

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Here we report quantitative data on the amount of single-walled carbon nanotubes that can be suspended with oligodeoxynucleotides in aqueous buffer, together with rate constants for the thermal denaturation of the resulting DNA-nanotube complexes at elevated temperatures. Sequence motifs d(GT)(n) and d(AC)(n) with n=2, 3, 5, 10, 20, or 40 were employed, both individually and as equimolar mixtures of the complementary strands. Unexpectedly, the greatest suspending efficiency was found for the mixture of short, complementary oligonucleotides d(GT)(3) and d(AC)(3). Unlike the suspending efficiency, the kinetic stability of the nanotube suspensions increases with increasing chain length of the DNA, with half life times of > 25 h at 90 degrees C for the complexes of the longest strands. Our results identify a new, unexpected optimum in DNA sequence space for suspending carbon nanotubes. They also demonstrate that suspending, power depends on the presence of complementary strands. Exploratory assays suggest that nanotubes can be deposited site-selectively from suspensions formed with short DNA sequences.
机译:在这里,我们报告了可与寡脱氧核苷酸悬浮在水性缓冲液中的单壁碳纳米管数量的定量数据,以及在升高的温度下所得DNA-纳米管复合物热变性的速率常数。使用n = 2、3、5、10、20或40的序列基序d(GT)(n)和d(AC)(n),既单独使用也作为互补链的等摩尔混合物使用。出乎意料的是,发现短的互补寡核苷酸d(GT)(3)和d(AC)(3)的混合物具有最大的悬浮效率。与悬浮效率不同,纳米管悬浮液的动力学稳定性随DNA链长度的增加而增加,对于最长链的复合物,其90°C下的半衰期> 25 h。我们的结果确定了用于悬浮碳纳米管的DNA序列空间中新的,出乎意料的最佳选择。他们还证明了悬浮力取决于互补链的存在。探索性试验表明,纳米管可以从短DNA序列形成的悬浮液中定点沉积。

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