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首页> 外文期刊>Psychosomatic Medicine: Journal of the American Psychosomatic Society >Reduced primary antibody responses in a genetic animal model of depression.
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Reduced primary antibody responses in a genetic animal model of depression.

机译：在抑郁症的遗传动物模型中减少的一抗反应。

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OBJECTIVE: Clinical depression is associated with multiple abnormalities of immune function, including reduced virus-specific responses. This study tested the hypothesis that the Flinders Sensitive Line (FSL) rat, a promising genetic animal model of depression, would exhibit reductions in antigen-specific primary antibody responses to immunization. METHODS: FSL (N = 13) and control Flinders Resistant Line (FRL; N = 14) rats were immunized with the protein antigen keyhole limpet hemocyanin (KLH; 300 microg/kg), and KLH-specific immunoglobulin (Ig)M, IgG, IgG1, and IgG2a responses were measured before and 3, 5, 7, 11, and 14 days after immunization. In separate experiments, production of interferon-gamma (IFN-gamma) by cells from naive and KLH-immunized animals was measured in vitro to determine whether strain differences in antibody production might be associated with differential production of this regulatory cytokine. RESULTS: KLH-specific production of IgM (p <.01) and IgG2a (p <.05) was significantly reduced in the FSL rats compared with the FRL controls. There were no strain differences in IgG or IgG1 production. Although IFN-gamma production between the two strains was similar in naive animals, cells from KLH-immunized FSL rats produced significantly less IFN-gamma when stimulated with KLH in vitro than cells from KLH-immunized FRL controls (p =.01). CONCLUSIONS: This study extends previous reports of altered immune function in the FSL rats to include reduced in vivo antigen-specific antibody responses. Moreover, diminished production of IFN-gamma by KLH-primed lymphocytes may contribute to lower antibody production in these animals. Collectively, these data suggest deficiencies in type 1 T-helper cell-mediated immunity in the FSL rats.
机译：目的：临床抑郁症与多种免疫功能异常有关，包括减少的病毒特异性反应。这项研究检验了以下假设：弗林德斯敏感线（FSL）大鼠是一种很有前途的抑郁症遗传动物模型，其对免疫的抗原特异性一抗反应将减少。方法：用蛋白抗原匙孔戚血蓝蛋白（KLH; 300 microg / kg）和KLH特异性免疫球蛋白（Ig）M，IgG免疫FSL（N = 13）和对照抗弗林德氏菌系（FRL; N = 14）大鼠。在免疫前和免疫后3、5、7、11和14天测量IgG1，IgG1和IgG2a应答。在单独的实验中，对来自幼稚和KLH免疫动物的细胞产生的干扰素-γ（IFN-γ）进行了体外测量，以确定抗体产生的菌株差异是否可能与该调节性细胞因子的差异产生有关。结果：与FRL对照相比，FSL大鼠的KLH特异性IgM（p <.01）和IgG2a（p <.05）产生明显减少。 IgG或IgG1的产生没有菌株差异。尽管两种菌株之间的IFN-γ产生在幼稚动物中是相似的，但是在体外用KLH刺激时，来自KLH免疫的FSL大鼠的细胞产生的IFN-γ明显低于来自KLH免疫的FRL对照的细胞（p = .01）。结论：这项研究扩展了FSL大鼠免疫功能改变的先前报道，包括体内抗原特异性抗体应答降低。此外，KLH引发的淋巴细胞减少的IFN-γ产生可能有助于降低这些动物的抗体产生。总的来说，这些数据表明FSL大鼠的1型T辅助细胞介导的免疫缺陷。

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