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首页> 外文期刊>Chemistry: A European journal >Thermally responsive supramolecular nanomeshes for on/off switching of the rotary motion of F-1-ATPase at the single-molecule level
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Thermally responsive supramolecular nanomeshes for on/off switching of the rotary motion of F-1-ATPase at the single-molecule level

机译：热响应超分子纳米网用于单分子水平上F-1 ATPase旋转运动的开/关切换

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摘要

The artificial regulation of protein functions is essential for the realization of protein-based soft devices, because of their unique functions conducted within a nano-sized molecular space. We report that self-assembled nanomeshes comprising heat-responsive supramolecular hydrogel fibers can control the rotary motion of an enzyme-based biomotor (F-1-ATPase) in an on/off manner at the single-molecule level. Direct observation of the interaction of the supramolecular fibers with a microbead unit tethered to the F-1-ATPase and the clear threshold in the size of the bead required to stop ATPase rotation indicates that the bead was physically blocked so as to stop the rotary motion of ATPase. The temperature-induced formation and collapse of the supramolecular nanomesh can produce or destroy, respectively, the physical obstacle for ATPase so as to control the ATPase motion in an off/on manner. Furthermore, this switching of the F-1-ATPase motion could be spatially restricted by using a microheating device. The integration of biomolecules and hard materials, interfaced with intelligent soft materials such as supramolecular hydrogels, is promising for the development of novel semi-synthetic nano-biodevices.
机译：蛋白质功能的人工调节对于基于蛋白质的软设备的实现至关重要，因为它们在纳米大小的分子空间中进行了独特的功能。我们报告说，包括热响应性超分子水凝胶纤维的自组装纳米网格可以在单分子水平上以开/关的方式控制基于酶的生物马达（F-1-ATPase）的旋转运动。直接观察超分子纤维与束缚在F-1-ATPase上的微珠单元的相互作用以及停止ATPase旋转所需的珠子大小的清晰阈值，表明该珠子已被物理阻滞，从而停止了旋转运动ATPase。温度诱导的超分子纳米网的形成和破坏可以分别产生或破坏ATPase的物理障碍，从而以开/关的方式控制ATPase的运动。此外，通过使用微加热装置可以在空间上限制F-1-ATPase运动的这种转换。生物分子和硬质材料的结合，以及与超分子水凝胶等智能软质材料的结合，对于新型半合成纳米生物装置的开发是有前途的。

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