• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >On the Bioactive Conformation of the Bhodopsin Chromophore: Absolute Sense of Twist around the 6-s-cis Bond
【24h】

On the Bioactive Conformation of the Bhodopsin Chromophore: Absolute Sense of Twist around the 6-s-cis Bond

机译：关于视紫红质生色团的生物活性构象：围绕6-s-顺式键的绝对扭曲感。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Incubation of opsin with synthetic 6-s-locked retinoids 2a and 2b only led to pigment formation from the alpha-locked 2a, the CD spectrum of which was similar to that of native rhodopsin (Rh). This establishes that the 6-s-bond of the chromophore in rhodopsin is cis, and that its helicity is negative. Earlier cross-linking studies showed that the 11-cis to all-trans photoisomerization occurring in the batho-Rh to lumi-Rh conversion induces a flip over of the side carrying the ring moiety. The GTP-binding assay of pigment Rh-(2a), incorporating retinal analogue 2a, has shown that its activity is 80% that of the native pigment. That is, the overall conformation around the 6-s bond is retained in the steps leading to G-protein activation.
机译：将视蛋白与合成的6-s锁定类视色素2a和2b一起孵育只会导致α锁定的2a形成色素，其CD光谱类似于天然视紫红质（Rh）。这表明视紫红质中发色团的6-s键为顺式，且其螺旋度为负。较早的交联研究表明，在batho-Rh到lumi-Rh转化中发生的11-顺式到全反式光异构化引起了带有环部分的一侧的翻转。结合了视网膜类似物2a的色素Rh-（2a）的GTP结合测定表明，其活性是天然色素的80％。即，在导致G蛋白活化的步骤中保留了6-s键周围的整体构象。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号