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首页> 外文期刊>Chemistry: A European journal >Carboxylate anion diminishes chloride transport through a synthetic, self-assembled transmembrane pore
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Carboxylate anion diminishes chloride transport through a synthetic, self-assembled transmembrane pore

机译：羧酸根阴离子减少了氯离子通过合成的，自组装的跨膜孔的传输

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摘要

Six amphiphilic heptapeptides with the structure (C18H37)(2)NCOCH2OCH2CO-(Gly)(3)-Pro-(Gly)(n)-(Glx)- (Gly)(m)-O(CH2)(6)CH3, in which Glx represents glutamic acid or its benzyl ester and n + m = 2, have been studied. In addition, the glutamate residue in the GGGPGGE sequence was esterified by fluorescent 1-pyrenemethanol. These compounds insert into phospholipid bilayers and form anion-conducting pores. Hill plots based on carboxyfluorescein release indicate that the pores are at least dimeric. Studies that involved ion-selective electrode techniques showed that transport of chloride varied with the position of glutamate within the peptide chain and whether glutamic acid was present as the free acid or its benzyl ester. Chloride transport activity was significantly higher for the glutamate esters than for free carboxylates irrespective of the glutamate position. Activity was highest when the glutamate residue in similar to(GlY)(3)-Pro-(Xxx)(3)similar to was closest to the C terminus of the peptide. A fluorescent pyrene residue was introduced to probe the aggregation state of the amphiphile. The selectivity of the pore for Cl- over K+ was maintained even when the carboxylate anion was present within it. Complexation of Cl- by the ionophoric peptides was confirmed by negative ion mass spectrometry. Planar bilayer voltage clamp experiments confirmed that pores with more than one conductance state may form in these dynamic, self-assembled pores.
机译：六个结构（C18H37）（2）NCOCH2OCH2CO-（Gly）（3）-Pro-（Gly）（n）-（Glx）-（Gly）（m）-O（CH2）（6）CH3的七肽已经研究了其中Glx代表谷氨酸或其苄基酯且n + m ＝ 2的化合物。另外，GGGPGGE序列中的谷氨酸残基被荧光的1-py基甲醇酯化。这些化合物插入磷脂双层中并形成传导阴离子的孔。基于羧基荧光素释放的希尔图表明，孔至少为二聚体。涉及离子选择电极技术的研究表明，氯的​​转运随肽链中谷氨酸的位置以及谷氨酸是否以游离酸或其苄基酯存在而变化。谷氨酸酯的氯离子转运活性明显高于游离羧酸盐，而与谷氨酸位置无关。当与（G1Y）（3）-Pro-（Xxx）（3）相似的谷氨酸残基最接近肽的C末端时，活性最高。引入荧光pyr残基以探测两亲物的聚集状态。即使当其中存在羧酸根阴离子时，也保持了孔对Cl-相对于K +的选择性。通过负离子质谱法证实了离子载体肽与Cl-的络合。平面双层电压钳实验证实，在这些动态的自组装孔中可能会形成具有一种以上电导状态的孔。

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