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A Near-Infrared Fluorescence-Based Optical Thermosensor

机译：基于近红外荧光的光学热传感器

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摘要

Fluorescence-based sensors have attracted a great deal of attention because they allow for the specific, rapid and sensitive detection of nonfluorescent analytes by simple emission enhancement or quenching without special instrumentation.[1] Various fluorescent sensors have been developed to more accurately detect and image various types of analytes,including biomolecules, organic molecules, pH and ions. However, fluorescent sensors capable of detecting changes in environmental conditions such as temperature have rarely been described, even though the development of a fluorescence-based thermosensor is desired in industrial testing and manufacturing and also in many biomedical diagnostic and treatment processes, such as measuring induced hyperthermia around tumors and inflammation.[2] Especially, fluorescence-based optical thermosensors are advantageous compared to contact temperature sensors in monitoring temperature within microanometer range (e.g., biological cells or nano/micro devices), where it is physically difficult to connect, since there is no contact with the medium in the measurement process.[3] Recently, fluorescent thermosensors derived from temperature-responsive organic molecules and polymers, such as poly(N-isopropyl acrylamide), have been studied and provide easy solutions for temperature monitoring on the basis of fluorescence emission intensity.[4, 5] However, there are no reports of fluorescent thermosensors capable of providing selective fluorescence visualization at a broad temperature range in real-time. This application is limited because these sensors only display luorescence ratiometry of emissions, show modest fluorescence changes at a narrow temperature range, and therefore provide insufficient resolution.
机译：基于荧光的传感器引起了极大的关注，因为它们允许通过简单的发射增强或淬灭而无需特殊仪器来特异性，快速和灵敏地检测非荧光分析物。[1]已经开发出各种荧光传感器以更准确地检测和成像各种类型的分析物，包括生物分子，有机分子，pH和离子。然而，即使在工业测试和制造以及许多生物医学诊断和治疗过程中，例如测量诱导温度，需要开发基于荧光的热传感器，也很少描述能够检测环境条件（例如温度）变化的荧光传感器。肿瘤和炎症周围的体温过高。[2]尤其是，基于荧光的光学热传感器与接触式温度传感器相比，在监视物理上难以连接的微/纳米范围（例如生物细胞或纳米/微设备）内的温度方面具有优势，因为它与介质没有接触在测量过程中。[3]近年来，已经研究了由对温度敏感的有机分子和聚合物（例如聚（N-异丙基丙烯酰胺））衍生的荧光热传感器，并基于荧光发射强度为温度监控提供了简便的解决方案。[4，5]尚无关于能够在宽温度范围内实时提供选择性荧光可视化的荧光热传感器的报道。此应用受到限制，因为这些传感器仅显示发射的荧光比率，在狭窄的温度范围内显示适度的荧光变化，因此分辨率不足。

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