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首页> 外文期刊>Chemistry: A European journal >Rhodamine Spiroamides for Multicolor Single-Molecule SwitchingFluorescent Nanoscopy
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Rhodamine Spiroamides for Multicolor Single-Molecule SwitchingFluorescent Nanoscopy

机译：罗丹明螺酰胺用于多色单分子开关荧光纳米显微镜

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The design, synthesis, andevaluation of new rhodamine spiro-amides are described. These moleculeshave applications in optical nanoscopybased on random switching of the fluo-rescent single molecules. The newmarkers may be used in (co)localiza-tion studies of various objects and their(mutual) positions and shape can bedetermined with a precision of a fewtens of nanometers. Multicolor stain-ing, good photoactivation, a largenumber of emitted photons, and selec-tive chemical binding with amino orthiol groups were achieved due to thepresence of various functional groups on the rhodamine spiroamides. Rigi-dized sulfonated xanthene fragmentfused with six-membered rings, N,N'-bis(2,2,2-trifluoroethyl) groups, and acombination of additional doublebonds and sulfonic acid groups withsimple aliphatic spiroamide residueprovide multicolor properties and im-prove performance of the rhodaminespiroamides in photoactivation andbioconjugation reactions. Having both essential parts of the photoswitchableassembly—the switching and the fluo-rescent (reporter) groups—combinedin one chemical entity make this ap-proach attractive for further develop-ment. A series of rhodamine spiro-amides is presented along with charac-terizations of their most relevant prop-erties for application as fluorescentprobes in single-molecule switchingand localization microscopy. Opticalimages with resolutions on the nano-meter scale illustrate the potential ofthe labels in the colocalization of bio-logical objects and the two-photon acti-vation technique with opticalsectioning.
机译：描述了新罗丹明螺酰胺的设计，合成和评价。这些分子基于荧光单个分子的随机转换在光学纳米技术中具有应用。新标记可用于各种物体的（共）定位研究，并且它们的（相互）位置和形状可以以几十纳米的精度确定。由于若丹明螺环酰胺上存在各种官能团，实现了多色染色，良好的光活化，大量发射的光子以及与氨基或硫醇基团的选择性化学结合。带有六元环，N，N'-双（2,2,2-三氟乙基）基团的Rigi修饰的磺化x吨片段与简单的脂肪族螺酰胺残基组合的其他双键和磺酸基团提供了多色性质，并改善了罗丹明螺酰胺在光活化和生物共轭反应中的作用。具有光开关组件的两个基本部分-开关和荧光（报告）基团-结合在一个化学实体中，使该方法对于进一步开发具有吸引力。介绍了一系列罗丹明螺酰胺及其最相关特性的表征，以用作荧光探针在单分子转换和定位显微镜中的应用。具有纳米级分辨率的光学图像说明了标签在生物物体和带光学切片的双光子活化技术共定位中的潜力。

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