• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Recognition of DNA three-way junctions by metallosupramolecular cylinders: Gel electrophoresis studies
【24h】

Recognition of DNA three-way junctions by metallosupramolecular cylinders: Gel electrophoresis studies

机译：金属超分子圆柱体识别DNA三向连接：凝胶电泳研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The interaction of metallosupramolecular cylinders with DNA three-way junctions has been studied by gel electrophoresis. A recent X-ray crystal structure of a palindromic oligonucleotide forming part of a complex with such a cylinder revealed binding at the heart of a three-way junction structure. The studies reported herein confirm that this is not solely an artefact of crystallisation and reveal that this is a potentially very powerful new mode of DNA recognition with wide scope. The cylinders are much more effective at stabilizing three-way junctions than simple magnesium dications or organic or metallo-organic tetracations, with the M cylinder enantiomer being more effective than P. The recognition is not restricted to three-way junctions formed from palindromic DNA with a central AT step at the junction; non-palindromic three-way junctions and those with GC steps are also stabilised. The cylinder is also revealed to stabilise other Y-shaped junctions, such as that formed at a fraying point in duplex DNA (for example, a replication fork), and other DNA three-way junction structures, such as those containing impaired nucleotides, perhaps by opening up this structure to access the central cavity.
机译：通过凝胶电泳研究了金属超分子圆柱体与DNA三向连接的相互作用。与这种圆柱体形成复合体一部分的回文寡核苷酸的最新X射线晶体结构揭示了在三向连接结构的核心处的结合。本文报道的研究证实，这不仅是结晶的人工产物，而且揭示了这是具有广泛范围的潜在的非常强大的DNA识别新模式。与简单的镁离子或有机或金属有机四阳离子相比，圆柱体在稳定三向连接方面更有效，M圆柱对映体比P更有效。识别不限于由回文DNA与交界处的中央AT台阶;非回文三通路口和带有GC步骤的路口也已稳定。还显示了圆柱体可以稳定其他Y形连接，例如在双链DNA（例如复制叉）的磨损点形成的连接，以及其他DNA三向连接结构，例如那些含有受损核苷酸的连接通过打开此结构以进入中央腔体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号