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首页> 外文期刊>Chemistry: A European journal >Aggregation behavior and dynamics of synthetic amphiphiles that self-assemble to anion transporters
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Aggregation behavior and dynamics of synthetic amphiphiles that self-assemble to anion transporters

机译：自组装为阴离子转运蛋白的合成两亲物的聚集行为和动力学

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摘要

The amphiphilic heptapeptides-referred to as synthetic anion transporters (SATs)-mediate chloride transport in planar lipid bilayer membranes, synthetic liposomes, and mammalian cells. The SATs described have the general formula (R2NCOCH2OCH2CO)-N-1-(Gly)(3)-Pro-(Gly)(3)-OR2. Substitution at R-1 and R-2 with various aliphatic or aromatic groups alters the ability of SATs to transport chloride through a phospholipid bilayer membrane. Despite extensive structure-activity relationship studies concerning Cl--mediated transport by SATs, relatively little was known about the mechanism of insertion and pore-formation in the membrane. In the current study, the mechanistic behavior of SATs was investigated in aqueous solution and at the air-water interface. In the latter case, Langmuir trough studies and Brewster angle microscopy (BAM) revealed the extent of monolayer stability and organization for SATs. Dynamic light scattering and transmission electron microscopy (TEM) confirmed these results and defined the aggregation behavior of SATs in solution. SAT derivatives that showed low chloride transport activity organized into stable monolayers at the air-water interface, while more active SATs formed less stable monolayers. The relationship between intermolecular organization of SATs and pore-formation in the membrane is discussed along with its implications for chloride transport.
机译：两亲性七肽-称为合成阴离子转运蛋白（SATs）-介导氯在平面脂质双层膜，合成脂质体和哺乳动物细胞中的转运。所描述的SAT具有通式（R2NCOCH2OCH2CO）-N-1-（Gly）（3）-Pro-（Gly）（3）-OR2。用各种脂族或芳族基团取代R-1和R-2会改变SATs通过磷脂双层膜转运氯化物的能力。尽管对SATs介导的Cl介导的转运进行了广泛的结构-活性关系研究，但对膜中插入和孔形成的机理知之甚少。在当前的研究中，研究了SATs在水溶液中和在空气-水界面处的力学行为。在后一种情况下，Langmuir槽研究和布鲁斯特角显微镜（BAM）揭示了SAT的单层稳定性和组织程度。动态光散射和透射电子显微镜（TEM）证实了这些结果，并定义了溶液中SAT的聚集行为。 SAT衍生物显示出较低的氯化物传输活性，在空气-水界面处组织成稳定的单层，而活性更高的SAT则形成了较不稳定的单层。讨论了SATs的分子间组织与膜中孔形成之间的关系及其对氯化物运输的影响。

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