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首页> 外文期刊>Chemistry: A European journal >Switchable cucurbituril-bipyridine beacons
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Switchable cucurbituril-bipyridine beacons

机译：可切换的葫芦素联吡啶信标

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摘要

4-Aminobipyridine derivatives form strong inclusion complexes with cucurbit[6]uril, exhibiting remarkably large enhancements in fluorescence intensity and quantum yields. The remarkable complexation-induced pK_a shift (ΔpK_a =3.3) highlights the strong charge-dipole interaction upon binding. The reversible binding phenomenon can be used for the design of switchable beacons that can be incorporated into cascades of binding networks. This concept is demonstrated herein by three different applications: 1) a switchable fluorescent beacon for chemical sensing of transition metals and other ligands; 2) direct measurement of binding constants between cucurbit[6]uril and various nonfluorescent guest molecules; and 3) quantitative monitoring of biocatalytic reactions and determination of their kinetic parameters. The latter application is illustrated by the hydrolysis of an amide catalyzed by penicillin G acylase and by the elimination reaction of a b-cabamoyloxy ketone catalyzed by aldolase antibody 38C2.
机译：4-氨基联吡啶衍生物与葫芦[6] uril形成强的包合物，在荧光强度和量子产率上有显着提高。显着的络合诱导的pK_a位移（ΔpK_a= 3.3）突出了结合后强烈的电荷-偶极相互作用。可逆绑定现象可用于可切换信标的设计，该可切换信标可并入绑定网络的级联中。本文通过三种不同的应用展示了该概念：1）用于化学感应过渡金属和其他配体的可切换荧光信标; 2）直接测量葫芦[6]尿素与各种非荧光客体分子之间的结合常数; 3）定量监测生物催化反应及其动力学参数。通过青霉素G酰基转移酶催化的酰胺的水解和醛缩酶38C2催化的β-氨基甲酰氧基酮的消除反应来说明后一种应用。

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