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首页> 外文期刊>Biomaterials >Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis.
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Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis.

机译：CD69活化抗原在髋关节假体患者淋巴细胞上的表达。

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The aim of the study was to evaluate the sensitization to metals in patients with Co-Cr hip prosthesis. Peripheral blood mononuclear cells (PBMC) were collected from 14 healthy donors and three groups of patients: 10 candidates for primary total joint replacements, 11 patients with well-fixed implant and 13 patients with aseptic loosening of the hip prosthesis. PBMCs were cultured with the metal ions employed for implant manufacturing and the expression of CD69 activation antigen on CD3/T lymphocytes was detected by flow cytometry. Chromium extract increased significantly the expression of CD3/CD69 phenotype in patients with loosening of hip prosthesis. The chromium-induced 'activation index' was higher in patients with loosening of hip prosthesis than in healthy donors and in pre-implant patients. The cobalt-stimulated PBMC of patients with either well-fixed or loosened prosthesis had an 'activation index' significantly higher than healthy donors. The activation index values were used to graduate the PBMC-response as 'normal' (> or = 0.9 and < 2), 'low' (< 0.9) and 'high' (> or = 2): an high-activation index was observed only in chromium-exposed PBMC of patients with prosthesis. Our data show that chromium released from orthopedic implants could be responsible for the lymphocyte sensitization and flow cytometry is an easy and reliable method for monitoring the hypersensitivity state in patients with metal prostheses. Activated lymphocytes in the peri-implant tissue are likely to elicit a localized immune response and contribute to maintain the inflammatory process evolving in the implant failure.
机译：这项研究的目的是评估Co-Cr髋关节假体患者对金属的敏感性。从14位健康捐献者和3组患者中收集外周血单个核细胞（PBMC）：10位主要全关节置换患者，11例植入物固定良好的患者和13例髋关节假体无菌性松动的患者。用用于制造植入物的金属离子培养PBMC，并通过流式细胞术检测CD69激活抗原在CD3 / T淋巴细胞上的表达。铬提取物可显着提高髋关节假体松弛患者CD3 / CD69表型的表达。髋关节假体松弛患者中铬引起的“活化指数”高于健康供体和植入前患者。假体固定或松弛的患者用钴刺激的PBMC的“激活指数”显着高于健康供体。激活指数值用于将PBMC响应分级为“正常”（>或= 0.9和<2），“低”（<0.9）和“高”（>或= 2）：高激活指数为仅在假体患者的暴露于铬的PBMC中观察到。我们的数据表明，从整形外科植入物中释放的铬可能导致淋巴细胞致敏，而流式细胞术是监测金属假体患者超敏状态的一种简单而可靠的方法。植入物周围组织中的活化淋巴细胞很可能引起局部免疫反应，并有助于维持植入物衰竭发展中的炎症过程。

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