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首页> 外文期刊>Chemistry: A European journal >Gold Nanolabels for New Enhanced Chemiluminescence Immunoassay of Alpha-Fetoprotein Based on Magnetic Beads
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Gold Nanolabels for New Enhanced Chemiluminescence Immunoassay of Alpha-Fetoprotein Based on Magnetic Beads

机译：金纳米标签用于基于磁珠的α-甲胎蛋白的新型增强化学发光免疫测定

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摘要

A novel and sensitive chemiluminescence immunoassay (CLIA) has been developed by employing a new chemiluminescence (CL) enhancer, bromophenol blue (BPB), for the determination of alpha-fetoprotein (AFP) based on magnetic beads (MBs) and colloidal gold nanoparticles (AuNPs) modified with HRP-labeled anti-AFP antibodies. BPB. as a chemical indicator, was found to act as a novel and highly signal enhancer of the peroxidase-catalyzed CL reaction of lurninol with hydrogen peroxide. After optimizing the CL reaction conditions, this new luminol-H2O2-HRP-BPB CL system was applied to a sandwich-type CLIA based on the magnetic separation and the amplification feature of AuNPs as HRP labels. A linear range was obtained when the concentrations of AFP were from 0.1. to 5.0 ng mL(-1) (R=0.9997) with the detection limit of 0.01 ngmL(-1) (3 sigma), which is one order of magnitude lower than that obtained without using AuNPs, and much lower than that typically achieved by ELISA. The present method was successfully applied to the determination of AFP in human serum samples. The results indicated that this proposed protocol could be quite promising for the application in immunoassays.
机译：通过使用新型化学发光（CL）增强剂溴酚蓝（BPB），开发了一种新颖且灵敏的化学发光免疫测定法（CLIA），用于测定基于磁珠（MBs）和胶体金纳米颗粒的甲胎蛋白（AFP）（用HRP标记的抗AFP抗体修饰的AuNPs。 BPB。作为化学指示剂，已发现其作为过氧化物酶催化的鲁尼诺醇与过氧化氢的CL反应的新型且高度信号增强剂。在优化CL反应条件后，基于磁性分离和AuNPs作为HRP标记的扩增特征，将这种新的鲁米诺-H2O2-HRP-BPB CL系统应用于三明治型CLIA。当AFP的浓度为0.1时，获得线性范围。到5.0 ng mL（-1）（R = 0.9997），检出限为0.01 ngmL（-1）（3 sigma），这比不使用AuNPs获得的检测限低一个数量级，并且比通常实现的检测限低得多通过ELISA。本方法已成功应用于人血清样品中AFP的测定。结果表明，该提议的方案对于在免疫测定中的应用可能是非常有前途的。

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