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首页> 外文期刊>Chemistry: A European journal >Enzyme Fingerprints of Activity, and Stereo- and Enantioselectivity from Fluorogenic and Chromogenic Substrate Arrays
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Enzyme Fingerprints of Activity, and Stereo- and Enantioselectivity from Fluorogenic and Chromogenic Substrate Arrays

机译：酶指纹的活性，以及​​从荧光和生色底物阵列的立体和对映选择性。

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摘要

A series of stereochemically and structurally diverse fluorogenic and chromogenic substrates for hydrolytic enzymes has been synthesized and used to characterize enzyme activity profiles of esterases, lipases, proteases, peptidases, phosphatases, and epoxide hydrolases. The substrates used are aprticularly resilient to nonspecific reactions due to their mechanism of activation. The activities recorded with the individual substrates are therefore remarkably reproducible, and enable us to use the overall pattern of activity as a specific fingerprint for the enzyme sample. Fingerprints of activity, and enantio- and stereoselectivity are displayed as arrays of color-scale squares that are easily analyzed visually. Such fingerprints might the useful for quality control, enzyme discovery, and possibly for addressing the issue of functional convergence in enzymes.
机译：已经合成了用于水解酶的一系列立体化学和结构上不同的荧光和生色底物，并用于表征酯酶，脂肪酶，蛋白酶，肽酶，磷酸酶和环氧化物水解酶的酶活性。由于它们的活化机理，所用的底物对非特异性反应特别有弹性。因此，用单个底物记录的活性是非常可重现的，并使我们能够使用总体活性模式作为酶样品的特定指纹。活动的指纹以及对映和立体选择性均显示为易于在视觉上分析的色标方块阵列。这样的指纹对于质量控制，酶发现以及可能解决酶功能收敛的问题可能有用。

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