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A System for Measuring Cell Division Patterns of Early Caenorhabditis elegans Embryos by Using Image Processing and Object Tracking

机译:利用图像处理和目标跟踪测量早期秀丽隐杆线虫胚胎细胞分裂模式的系统

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摘要

Cell division patterns provide crucial information for understanding the mechanisms of development of multicellular organisms. These patterns are measured manually by long-term microscopic observation or by studying large numbers of images taken by using time-lapse microscope systems. Because manual measurement limits the objectivity and productivity of measurement, the comprehensive cell division pattern analysis of gene knockdown embryos has been difficult. Here, we present a system that automatically measures the cell division pattern of Caenorhabditis elegans embryos from the 1- to 24-cell stages with high levels of objectivity and productivity. The system automatically detects the nuclei in a set of four-dimensional differential interference contrast microscope images of a C. elegans embryo by using image processing and measures the cell division pattern by tracking the detected nuclei over time. This system enables a comprehensive cell division pattern analysis of gene knockdown C. elegans embryos.
机译:细胞分裂模式为理解多细胞生物的发育机制提供了关键信息。这些图案是通过长期的显微镜观察或通过研究使用延时显微镜系统拍摄的大量图像来手动测量的。由于手动测量限制了测量的客观性和生产率,因此难以对基因敲除胚胎进行全面的细胞分裂模式分析。在这里,我们介绍了一个系统,该系统可以自动测量线虫秀丽隐杆线虫胚胎从1到24个细胞阶段的细胞分裂模式,具有很高的客观性和生产率。该系统通过使用图像处理,自动检测线虫胚胎的一组四维差分干涉对比显微镜图像中的细胞核,并通过随时间跟踪检测到的细胞核来测量细胞分裂模式。该系统能够对敲除秀丽隐杆线虫的胚胎进行全面的细胞分裂模式分析。

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