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首页> 外文期刊>Chemistry: A European journal >Ultrasensitive electrochemical analysis of two analytes by using an autonomous DNA machine that works in a two-cycle mode
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Ultrasensitive electrochemical analysis of two analytes by using an autonomous DNA machine that works in a two-cycle mode

机译：使用在两个循环模式下运行的自主DNA机器对两种分析物进行超灵敏的电化学分析

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摘要

We report a novel autonomous DNA machine for amplified electrochemical analysis of two DNAs. The DNA machine operates in a two-cycle working mode to amplify DNA recognition events; the working mode is assisted by two different nicking endonucleases (NEases). Two bio-barcode probes, a ZnS nanoparticle (NP)-DNA probe and a CdS NP-DNA probe, were used to trace two target DNAs. The detection system was based on a sensitive differential pulse anodic stripping voltammetry (DPASV) method for the simultaneous detection of Zn_(II) and Cd~(II) tracers, which were obtained by dissolving the two probes. Under the optimised conditions, detection limits as low as 5.6× 10 ~(-17) (3σ) and 4.1× 10~(-17) M (3σ) for the two target DNAs were achieved. It has been proven that the DNA machine system can simultaneously amplify two target DNAs by more than four orders of magnitude within 30 min at room temperature. In addition, in combination with an aptamer recognition strategy, the DNA machine was further used in the aptamer-based amplification analysis of adenosine triphosphate (ATP) and lysozyme. With the amplification of the DNA machine, detection limits as low as 5.6× 10 ~(-9) M (3σ) for ATP and 5.2× 10~(-13) M (3σ) for lysozyme were simultaneously obtained. The satisfactory determination of ATP and lysozyme in Ramos cells reveals the good selectivity and feasibility of this protocol. The DNA machine is a promising tool for ultrasensitive and simultaneous multianalysis because of its remarkable signal amplification and simple machine-like operation.
机译：我们报告了一种新颖的自主DNA机，用于扩增两个DNA的电化学分析。 DNA机器以两个周期的工作模式运行，以放大DNA识别事件。两种不同的切口内切核酸酶（NEases）辅助了工作模式。使用两种生物条形码探针，即ZnS纳米颗粒（NP）-DNA探针和CdS NP-DNA探针来追踪两个目标DNA。该检测系统基于灵敏的差分脉冲阳极溶出伏安法（DPASV），用于同时检测通过溶解两种探针获得的Zn_（II）和Cd〜（II）示踪剂。在优化的条件下，两个目标DNA的检出限分别低至5.6×10〜（-17）（3σ）和4.1×10〜（-17）M（3σ）。已经证明，DNA机器系统可以在室温下30分钟内同时扩增两个目标DNA超过四个数量级。此外，结合适体识别策略，DNA机器进一步用于基于适体的三磷酸腺苷（ATP）和溶菌酶的扩增分析。随着DNA机器的扩增，同时获得的ATP的检出限低至5.6×10〜（-9）M（3σ），而溶菌酶的检出限则低至5.2×10〜（-13）M（3σ）。对Ramos细胞中ATP和溶菌酶的满意测定揭示了该方案的良好选择性和可行性。 DNA机器具有出色的信号放大和简单的类似机器的操作，因此是用于超灵敏和同时进行多重分析的有前途的工具。

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