D-Altritol Nucleic Acids (ANA): Hybridisation Properties, Stability, and Initial Structural Analysis

Allart Brigitte; Khan Kamran; Rosemeyer Helmut

首页> 外文期刊>Chemistry: A European journal >D-Altritol Nucleic Acids (ANA): Hybridisation Properties, Stability, and Initial Structural Analysis

【24h】

D-Altritol Nucleic Acids (ANA): Hybridisation Properties, Stability, and Initial Structural Analysis

机译：D-麦芽糖醇核酸（ANA）：杂交性质，稳定性和初始结构分析

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Oligonucleotides composed of a phosphorylated D-altritol backbone with nucleobases introduced int he 2'-position, hybridise strongly and sequence-selectively with RNA in an antiparallel way. The order of hybridisation strength is: dsANA > ANA:RNA >ANA:DNA. Complexes between ANA and RNA or DNA are more stable than between HNA and natural oligonucleotides. Te dsANA hybrid is extremely stable. When compared with an identical dsDNA sequence, the #DELTA#T_m per modification is + 10.2deg C for a hexamer. CD spectral analysis indicates that ANA complexes are very similar to the A-form dsRNA duplex. ANA are stable in alaline medium up to pH 12 and, likewise, are not degraded in human serum.

机译：由磷酸化的D-麦芽糖醇骨架和2'-位引入的核碱基组成的寡核苷酸以反平行的方式与RNA强烈杂交并序列选择性地杂交。杂交强度的顺序为：dsANA> ANA：RNA> ANA：DNA。 ANA与RNA或DNA之间的复合物比HNA与天然寡核苷酸之间的复合物更稳定。 Te dsANA Hybrid非常稳定。当与相同的dsDNA序列进行比较时，对于六聚体，每个修饰的＃DELTA＃T_m为+ 10.2°C。 CD光谱分析表明，ANA复合物与A型dsRNA双链体非常相似。 ANA在高达pH 12的alaline培养基中稳定，并且在人血清中也不会降解。

著录项

来源
《Chemistry: A European journal》 |1999年第8期|共8页
作者
Allart Brigitte; Khan Kamran; Rosemeyer Helmut;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类化学;应用化学;
关键词
altritol nucleic acids; antisense agents; hexitol nucleic acids; oligonucleotides; thermal stability data;

机译：醛糖醇核酸;反义剂;己糖醇核酸;寡核苷酸;热稳定性数据;

相似文献

外文文献
中文文献
专利

1. D-Altritol Nucleic Acids (ANA): Hybridisation Properties, Stability, and Initial Structural Analysis [J] . Allart Brigitte, Khan Kamran, Rosemeyer Helmut Chemistry: A European journal . 1999,第8期

机译：D-麦芽糖醇核酸（ANA）：杂交性质，稳定性和初始结构分析
2. Design, synthesis, conformational analysis and nucleic acid hybridisation properties of thymidyl pyrrolidine-amide oligonucleotide mimics (POM) [J] . David T. Hickmanc, T. H. Samuel Tan, Jordi Morral, Organic & biomolecular chemistry . 2003,第19期

机译：胸苷基吡咯烷酰胺寡核苷酸模拟物（POM）的设计，合成，构象分析和核酸杂交特性
3. 2'-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry [J] . Ono T, Scalf M, Smith LM Nucleic Acids Research . 1997,第22期

机译：2'-氟修饰的核酸：聚合酶指导的合成，性质和稳定性，可通过基质辅助激光解吸/电离质谱法进行分析
4. In-cell NMR 法を用いた非標準構造を形成する核酸の構造安定性の解析(Analysis of the structural stability and dynamics of nucleic acidsforming non-canonical structures using in-cell NMR) [C] . ○阪本知樹, 山置佑大, 永田崇日本化学会春季年会 . 2020

机译：使用细胞NMR方法分析核酸形成非标结构的结构稳定性（Cell kMr中核酸结构稳定性和动力学的结构稳定性和动力学。使用细胞NMR）
5. Initial excited-state structural dynamics and damage kinetics of nucleic acid derivatives and a rhodopsin analogue. [D] . Sasidharanpillai, Swaroop. 2013

机译：核酸衍生物和视紫红质类似物的初始激发态结构动力学和破坏动力学。
6. 2-Fluoro modified nucleic acids: polymerase-directed synthesis properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry. [O] . T Ono, M Scalf, L M Smith 1997

机译：2-氟修饰核酸：聚合酶指导的合成性质和稳定性可通过基质辅助激光解吸/电离质谱法进行分析。
7. 2′β‐Fluoro‐Tricyclo Nucleic Acids (2′F‐tc‐ANA): Thermal Duplex Stability, Structural Studies, and RNase H Activation [O] . Alena Istrate, Adam Katolik, Andrei Istrate, 2017

机译：2'β-氟 - 三核核酸（2'F-TC-ANA）：热双相稳定性，结构研究和RNase H激活
8. Notes on the Determination of the Stability Constants of Copper-Complexes with Nucleic Acids and Nucleic Acid Derivatives [R] . Kaminski, L., Altmann, H., Kaindl, K. 1966

机译：关于用核酸和核酸衍生物测定铜配合物稳定常数的注记

D-Altritol Nucleic Acids (ANA): Hybridisation Properties, Stability, and Initial Structural Analysis

摘要

著录项

相似文献

相关主题

期刊订阅