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首页> 外文期刊>Peritoneal dialysis international: Journal of the International Society for Peritoneal Dialysis >Increased leukocyte rolling in newly formed mesenteric vessels in the rat during peritoneal dialysis.
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Increased leukocyte rolling in newly formed mesenteric vessels in the rat during peritoneal dialysis.

机译：腹膜透析期间大鼠新形成的肠系膜血管中白细胞滚动增加。

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OBJECTIVE: Long-term peritoneal dialysis (PD) is associated with the development of functional and structural alterations of the peritoneal membrane.The present study reports the effects of chronic exposure to PD fluid on mesenteric leukocyte-endothelium interactions, using intravital video microscopy. METHODS: Rats (n = 7) received 10 mL lactate-buffered 3.86% glucose-containing PD fluid daily during a 5-week period via a subcutaneously implanted mini access port that was connected via a catheter to the peritoneal cavity. In a first control group (n = 8), catheters were implanted but no fluid was instilled; a second control group (n = 8) remained untreated. The number of rolling and adherent leukocytes as well as blood flow and other fluid dynamic variables were analyzed in mesenteric postcapillary (diameter 10-25 mu) and collecting (diameter 26-40 mu) venules. Neovascularization was semiquantitatively assessed after inspection of video images and by light and electron microscopy. Using FITC-labeled albumin, microvascular leakage was examined. RESULTS: Rats exposed to PD fluid showed a more than twofold increase in the number of rolling leukocytes (p < 0.01); the number of adherent leukocytes was not changed. Furthermore, exposure to PD fluid induced severe neovascularization in rat mesentery. No microvascular leakage was observed in the various groups. The observed differences could not be explained by differences in systemic or local hemodynamic parameters or peripheral leukocyte counts, but is most likely associated with new vessel formation. CONCLUSIONS: Exposure of rat peritoneal membrane to conventional PD fluid for 5 weeks affected local leukocyte-endothelium interactions. In addition, severe angiogenesis was induced, whereas microvascular permeability remained unaltered.
机译：目的：长期腹膜透析（PD）与腹膜功能和结构改变有关。本研究利用活体视频显微镜报道了长期暴露于PD液对肠系膜白细胞-内皮相互作用的影响。方法：大鼠（n = 7）在5周内每天通过皮下植入的微型通道（通过导管连接到腹膜腔）接受10 mL乳酸盐缓冲的3.86％含葡萄糖的PD液。在第一个对照组（n = 8）中，植入了导管，但未滴入任何液体。第二对照组（n = 8）仍未接受治疗。在肠系膜后毛细血管（直径10-25微米）和收集（直径26-40微米）小静脉中分析了滚动白细胞和粘附白细胞的数量，以及血流量和其他流体动力学变量。在检查视频图像并通过光学和电子显微镜检查后，对新血管形成进行了半定量评估。使用FITC标记的白蛋白检查微血管渗漏。结果：暴露于PD液体的大鼠的滚动白细胞数目增加了两倍以上（p <0.01）;粘附的白细胞数目没有改变。此外，暴露于PD液可引起大鼠肠系膜严重新生血管形成。在各组中均未观察到微血管渗漏。观察到的差异无法通过全身或局部血液动力学参数或外周血白细胞计数的差异来解释，但很可能与新血管的形成有关。结论：将大鼠腹膜暴露于常规PD液中5周会影响局部白细胞与内皮的相互作用。另外，诱导了严重的血管生成，而微血管通透性保持不变。

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