• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Self-Organized Glycoclusters Along DNA:Effect of the Spatial Arrangement of Galactoside Residues on Cooperative Lectin Recognition
【24h】

Self-Organized Glycoclusters Along DNA:Effect of the Spatial Arrangement of Galactoside Residues on Cooperative Lectin Recognition

机译：DNA的自组织糖簇：半乳糖苷残基的空间排列对协同凝集素识别的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe herein the relationship between the spatial arrangement of self-organized galactose clusters and lectin recognition.beta-Galactose-modified deoxyuridine phosphoramidite was synthesized and applied to solid-phase synthesis to provide 18-,20-,and 22-mers of site-specifically galactosylated oligodeoxynucleotides (Gal-ODNs).These Gal-ODNs were self-organized through hybridization with the corresponding 18-,20-,and 22-mers of half-sliding complementary ODNs (hsc-ODNs) to give periodic galactoside clusters.The self-organization of ODNs was confirmed by size exclustion chromatography and gel electrophoresis.The binding of the Galclusters to the FITC-labeled RCA_(120) lectin was analyzed by monitoring the change in fluorescence intensity.The assembly of 20-mer Gal-ODN with the 20-mer hsc-ODN was strongly and cooperatively recognized by the lectin.The 18-mer assembly was bound more weakly and less cooperatively,and the 22-mer assembly was minimally bound to the lectin.RCA_(120) lectin recognized not only the density of galactoside residues,but also the spatial arrangement.The size of the Gal cluster was estimated from the association constant of Gal-ODN with hsc-ODN.The relationship between lectin-recognition and Gal-cluster size is also discussed.
机译：我们在此描述了自组织半乳糖簇的空间排列与凝集素识别之间的关系。合成了β-半乳糖修饰的脱氧尿苷亚磷酰胺并将其应用于固相合成，以提供18-，20-和22-mer位点-这些Gal-ODN通过与相应的18-，20-和22-mer的半滑动互补ODN（hsc-ODN）杂交而自我组织，从而形成周期性的半乳糖苷簇。这些半乳糖化的寡脱氧核苷酸（Gal-ODN）。通过尺寸排阻色谱和凝胶电泳证实了ODN的自组织。通过监测荧光强度的变化来分析Galclusters与FITC标记的RCA_（120）凝集素的结合.20-mer Gal-ODN的组装凝集素强烈和协同地识别20聚体hsc-ODN.18聚体的结合较弱且不那么协作，而22聚体的组件与凝集素的结合最少.RCA_（120） Gal簇的大小是根据Gal-ODN与hsc-ODN的缔合常数来估计的。凝集素识别与Gal簇大小之间的关系也是讨论过。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号