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首页> 外文期刊>Chemistry: A European journal >Development of a Quadruple Imaging Modality by Using Nanoparticles
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Development of a Quadruple Imaging Modality by Using Nanoparticles

机译：通过使用纳米粒子的四倍成像模式的发展。

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The combination of nano-technology with molecular imaging hasgreat potential for the development ofdiagnostics and therapeutics, and multi-modal imaging enables versatile appli-cations from cell tracking in animals toclinical applications. Herein, we reporta multimodal nanoparticle imagingsystem that is capable of concurrentfluorescence, bioluminescence, biolu-minescence resonance energy transfer(BRET), positron emission tomogra-phy (PET) and magnetic resonance(MR) imaging in vivo. A cobalt—ferritenanoparticle surrounded by rhodamine (MF) was conjugated with luciferase(MFB) and p-SCN—bn—NOTA (2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclo-nane-1,4,7-triacetic acid) followed by~(68)GaCl_3(magnetic-fluorescent-biolumi-nescent-radioisotopic particle, MFBR).Confocal microscopy revealed goodtransfection efficiency of MFB intocells and BRET was also observed inMFB. A good correlation among rhod-amine, luciferase, and ~(68)GaCl_3wasfound in MFBR, and the activities ofeach imaging modality increased dose-dependently with the amount ofMFBR in the C6 cells. In vivo opticalimages were acquired from the thighsof mice after intramuscular and subcu-taneous injections of MFBR-ladencells. MicroPET and MR imagesshowed intense radioactivity and ferro-magnetic intensities with MFBR-ladencells. The multimodal imaging strategycould be used as potential imagingtools to track cells.
机译：纳米技术与分子成像的结合在诊断和治疗学的发展上具有巨大的潜力，多模式成像使从动物细胞追踪到临床应用的多种应用成为可能。在本文中，我们报告了一种多模式纳米粒子成像系统，该系统能够在体内同时进行荧光，生物发光，生物发光共振能量转移（BRET），正电子发射体层摄影（PET）和磁共振（MR）成像。罗丹明（MF）包围的钴-铁氧体纳米粒子与萤光素酶（MFB）和p-SCN-bn-NOTA（2-（4-异硫氰酸根合苄基）-1,4,7-三氮杂环烷-1,4,7-用三乙酸），再用〜（68）GaCl_3（磁性荧光-生物发光-放射性同位素，MFBR）。共聚焦显微镜观察发现MFB具有良好的转染效率，在MFB中也观察到了BRET。在MFBR中发现了罗丹明胺，荧光素酶和〜（68）GaCl_3之间的良好相关性，并且每种成像方式的活性随C6细胞中MFBR的量呈剂量依赖性增加。在肌内和皮下注射MFBR-ladencells后，从小鼠大腿获取体内光学图像。 MicroPET和MR图像显示具有MFBR-ladendels的强放射性和铁磁强度。多峰成像策略可用作跟踪细胞的潜在成像工具。

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