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首页> 外文期刊>Chemistry: A European journal >Fabrication of a biocompatible and conductive platform based on a singlestranded DNA/graphene nanocomposite for direct electrochemistry and electrocatalysis
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Fabrication of a biocompatible and conductive platform based on a singlestranded DNA/graphene nanocomposite for direct electrochemistry and electrocatalysis

机译：基于单链DNA /石墨烯纳米复合材料的生物相容性和导电平台的制备，用于直接电化学和电催化

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摘要

A novel electrochemical platform was designed by combining the biocompatibility of single-stranded DNA (ss-DNA) and the excellent conductivity of graphene (GP). This nanocomposite (denoted as ss-DNA/GP) was first used as an electrode material for the immobilization and biosensing of redox enzymes. On the basis of electrostatic interactions, horseradish peroxidase (HRP) self-assembled with ssDNA/GP on the surface of a glassy carbon (GC) electrode to form an HRP/ss-DNA/GP/GC electrode. UV/ Vis and FTIR spectra were used to monitor the assembly process and indicated that the immobilized HRP on the ss-DNA/GP matrix retained its native structure well. A pair of stable and well-defined redox peaks of HRP with a formal potential of about -0.26 V (vs. Ag/AgCl) in a pH 7.0 phosphate buffer solution were obtained at the HRP/ss-DNA/GP/GC electrode; this demonstrates direct electron transfer between the immobilized HRP and the electrode. In addition, the modified electrode showed good electrocatalytic performance towards H_2O_2 with high sensitivity, wide linear range, and good stability. Accordingly, the ss-DNA/GP nanocomposite provides a novel and efficient platform for the immobilized redox enzyme to realize direct electrochemistry and has a promising application in the fabrication of third-generation electrochemical biosensors.
机译：通过结合单链DNA（ss-DNA）的生物相容性和优异的石墨烯电导率（GP）设计了一种新型的电化学平台。这种纳米复合材料（表示为ss-DNA / GP）首先被用作固定和生物传感氧化还原酶的电极材料。基于静电相互作用，辣根过氧化物酶（HRP）与ssDNA / GP自组装在玻璃碳（GC）电极的表面上，形成HRP / ss-DNA / GP / GC电极。 UV / Vis和FTIR光谱用于监测组装过程，并表明固定在ss-DNA / GP基质上的HRP很好地保留了其天然结构。在HRP / ss-DNA / GP / GC电极上获得了一对在pH 7.0磷酸盐缓冲溶液中具有约-0.26 V（vs. Ag / AgCl）形式电位的HRP稳定且定义明确的氧化还原峰;这证明了固定化HRP和电极之间的直接电子转移。另外，该修饰电极对H_2O_2具有良好的电催化性能，灵敏度高，线性范围宽，稳定性好。因此，ss-DNA / GP纳米复合材料为固定化的氧化还原酶实现直接电化学提供了一个新颖而有效的平台，在第三代电化学生物传感器的制造中具有广阔的应用前景。

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