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首页> 外文期刊>Chemistry: A European journal >Fabrication of nanoporous nanocomposites entrapping Fe_3O _4 magnetic nanoparticles and oxidases for colorimetric biosensing
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Fabrication of nanoporous nanocomposites entrapping Fe_3O _4 magnetic nanoparticles and oxidases for colorimetric biosensing

机译：包埋Fe_3O _4磁性纳米粒子和氧化酶的纳米多孔纳米复合材料的比色生物传感

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摘要

A nanostructured multicatalyst system consisting of Fe_3O _4 magnetic nanoparticles (MNPs) as peroxidase mimetics and an oxidative enzyme entrapped in large-pore-sized mesoporous silica has been developed for convenient colorimetric detection of biologically important target molecules. The construction of the nanocomposites begins with the incorporation of MNPs on the walls of mesocellular silica pores by impregnating Fe(NO _3)_3·9 H)_2O, followed by the immobilization of oxidative enzymes. Glutaraldehyde crosslinking was employed to prevent enzymes leaching from the pores and led to over 20 wt % loading of the enzyme. The oxidase in the nanocomposite generates H_2O_2 through its catalytic action for target molecules and subsequently activates MNPs to convert selected substrates into colored products. Using this strategy, two different biosensing systems were constructed employing glucose oxidase and cholesterol oxidase and their analytical capabilities were successfully verified by colorimetrically detecting the corresponding target molecules with excellent selectivity, sensitivity, reusability, and stability. Future potential applications of this technology range from biosensors to multicatalyst reactors.
机译：已开发出由Fe_3O_4磁性纳米颗粒（MNP）作为过氧化物酶模拟物和截留在大孔尺寸介孔二氧化硅中的氧化酶组成的纳米结构多催化剂系统，用于方便地比色检测生物学上重要的目标分子。纳米复合材料的构建始于通过浸渍Fe（NO _3）_3·9 H）_2O将MNP掺入介孔二氧化硅孔壁，然后固定氧化酶。戊二醛交联被用于防止酶从孔中浸出并导致酶的负载超过20重量％。纳米复合物中的氧化酶通过其对靶分子的催化作用生成H_2O_2，随后激活MNP以将选定的底物转化为有色产物。使用这种策略，使用葡萄糖氧化酶和胆固醇氧化酶构建了两种不同的生物传感系统，并且通过比色法检测相应的目标分子具有出色的选择性，灵敏度，可重复使用性和稳定性，从而成功验证了它们的分析能力。这项技术的未来潜在应用范围从生物传感器到多催化剂反应器。

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