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首页> 外文期刊>Chemistry: A European journal >Preparation and Characterization of DNA Films Induced by UV Irradiation
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Preparation and Characterization of DNA Films Induced by UV Irradiation

机译：紫外线辐照诱导的DNA膜的制备与表征

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摘要

Large amounts of DNA-enriched materials, such as samon milts and shellfish gonads, are discarded as industrial waste. We have been able to convert the discarded DNA to a useful material waste. We have been able to convert the discarded DNA to a useful material by preparing novel DNA films by UV irradiation. When DANa films were irradiated with UV light, the molecular wieght of DNA was greatly increased. The reaction was inhibited by addition of the radical scavenger galvinoxyl suggesting that the DNA polymerization with UV irradiation proceeded by a radical reaction. Although this UV-irradiated DNA film was water-insoluble and resistant to hydrolysis by nuclease, the structure of the DNA film in water was similar to non-irradiated DNA and maintained B-form structure. In addition, the UV-irradiated DNA film could effectively accumulate and condense harmful DNA-intercalating compoudns, such as ethidium bromide and acridine orange, from diluted aqueous solutions. The binding constant and exclusion number of ethidium bromide for UV-irradiated DAN were determined to be 6.8+-0.3X10~4M~(-1) and 1.6+-0.2, respectively; these values are consisted with reported results for nonirradiated DNA. The UV-irradiated DNA films have potential uses as a biomaterial filter for the removal of harmful DNA intercalating compounds.
机译：大量富含DNA的物质（如鲑鱼乳糜和贝类生殖腺）被作为工业废物丢弃。我们已经能够将丢弃的DNA转化为有用的材料废物。通过使用紫外线照射制备新型DNA膜，我们已经能够将废弃的DNA转化为有用的材料。当用紫外线照射DANa膜时，DNA的分子重量大大增加。通过加入自由基清除剂加尔维诺尔抑制了该反应，这表明在紫外线照射下的DNA聚合反应是通过自由基反应进行的。尽管该紫外线辐照的DNA膜不溶于水，并且耐核酸酶水解，但是水中的DNA膜的结构与未辐照的DNA相似，并保持了B型结构。此外，紫外线照射的DNA膜可以有效地积聚和浓缩稀释水溶液中有害的DNA嵌入化合物，例如溴化乙锭和a啶橙。溴化乙锭对紫外线辐照DAN的结合常数和排阻数分别为6.8 + -0.3X10〜4M〜（-1）和1.6 + -0.2。这些值与未辐照DNA的报告结果组成。紫外线照射的DNA膜具有潜在的用途，可以用作去除有害DNA嵌入化合物的生物材料过滤器。

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