• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Identifying the bond responsible for the fluorescence modulation in an amyloid fibril sensor
【24h】

Identifying the bond responsible for the fluorescence modulation in an amyloid fibril sensor

机译：识别负责淀粉样原纤维传感器中荧光调制的键

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An ultrafast intramolecular bond twisting process is known to be the responsible mechanism for the sensing activity of the extensively used amyloid fibril sensor thioflavin T (ThT). However, it is not yet known which one of the two possible single bonds in ThT is actually involved in the twisting process. To resolve this fundamental issue, two derivatives of ThT have been designed and synthesized and subsequently their photophysical properties have been studied in different solvents. It is understood from the present study that the rotation around the central C-C single bond, and not that around the C-N single bond, is primarily responsible for the sensor activity of ThT. Detailed viscosity-dependent fluorescence studies revealed that the ThT derivative with restricted C-N bond rotation acts as a better sensor than the derivative with free C-N bond rotation. The better sensory activity is directly correlated with a shorter excited-state lifetime. Results obtained from the photophysical studies of the ThT derivatives have also been supported by the results obtained from quantum chemical calculations.
机译：众所周知，超快分子内键加捻过程是广泛使用的淀粉样原纤维传感器硫黄素T（ThT）的传感活性的负责机制。但是，尚不清楚ThT中两个可能的单键中的哪一个实际上参与了加捻过程。为了解决这个基本问题，设计并合成了ThT的两种衍生物，随后在不同溶剂中研究了它们的光物理性质。从本研究中可以理解，围绕中心C-C单键而不是围绕C-N单键的旋转主要是ThT传感器的活动。详细的粘度依赖性荧光研究表明，与C-N键自由旋转的衍生物相比，C-N键旋转受到限制的ThT衍生物具有更好的传感性能。更好的感觉活性与较短的激发态寿命直接相关。从ThT衍生物的光物理研究获得的结果也得到了量子化学计算结果的支持。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号