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Expanded utility of the native chemical ligation reaction

机译：天然化学连接反应的扩展效用

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The post-genomic era heralds a multitude of challenges for chemists and biologists alike, with the study of protein functions at the heart of much research. The elucidation of protein structure, localization, stability, post-translational modifications, and protein interactions will steadily unveil the role of each protein and its associated biological function in the cell. The push to develop new technologies has necessitated the integration of various disciplines in science. Consequently, the role of chemistry has never been so profound in the study of biological processes. By combining the strengths of recombinant DNA technology, protein splicing, organic chemistry, and the chemoselective chemistry of native chemical ligation, various strategies have been successfully developed and applied to chemoselectively label proteins, both in vitro and in live cells, with biotin, fluorescent, and other small molecule probes. The site-specific incorporation of molecular entities with unique chemical functionalities in proteins has many potential applications in chemical and biological studies of proteins. In this article, we highlight recent progress of these strategies in several areas related to proteomics and chemical biology, namely, in vitro and in vivo protein biotinylation, protein microarray technologies for large-scale protein analysis, and live-cell bioimaging.
机译：基因组后时代预示着化学家和生物学家将面临许多挑战，蛋白质功能的研究是许多研究的核心。对蛋白质结构，定位，稳定性，翻译后修饰和蛋白质相互作用的阐明将稳步揭示每种蛋白质的作用及其在细胞中的相关生物学功能。推动新技术的发展已要求将科学的各个学科整合在一起。因此，化学的作用在生物学过程的研究中从未如此深刻。通过结合重组DNA技术，蛋白质剪接，有机化学和天然化学连接的化学选择化学的优势，已成功开发出各种策略，并利用生物素，荧光，和其他小分子探针。具有独特化学功能的分子实体在蛋白质中的位点特异性结合在蛋白质的化学和生物学研究中具有许多潜在的应用。在本文中，我们重点介绍了这些策略在与蛋白质组学和化学生物学相关的几个领域中的最新进展，这些领域包括体外和体内蛋白质生物素化，用于大规模蛋白质分析的蛋白质微阵列技术以及活细胞生物成像。

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