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首页> 外文期刊>Chemistry: A European journal >Supported bilayer lipid membrane arrays on photopatterned self-assembled monolavers
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Supported bilayer lipid membrane arrays on photopatterned self-assembled monolavers

机译：光图案化自组装单层胶上支持的双层脂质膜阵列

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This work demonstrates the use of photocleavable cholesterol derivatives to create supported bilayer lipid membrane arrays on silica. The photocleavable cholesteryl tether is attached to the surface by using the reaction of an amine-functionalized self-assembled monolayer (SAM) and the N-hydroxysuccinimide-based reagent 9. The resultant SAM contains an orthonitrobenzyl residue that can be cleaved by photolysis by using soft (365 nm) UV light regenerating the original amine surface, and which can be patterned using a mask. The photoreaction yield was approximate to 75% which was significantly higher than previously found for related ortho-nitrobenzyl photochemistry on gold substrates. The SAMs were characterized by means of contact angle measurements, ellipsometry and X-ray photoelectron spectroscopy. Patterned surfaces were characterized with SEM and AFM. After immersing the patterned surface into a solution containing small unilamellar vesicles of egg phosphatidylcholine (PC), supported lipid membranes were formed comprised of lipid bilayer over the amine functionalized "hydrophilic" regions and lipid monolayer over the cholesteryl "hydrophobic" regions. This was confirmed by fluorescence microscopy and AFM. FRAP studies yielded a lateral diffusion coefficient for the probe molecule of 0.14 +/- 0.05 mu m(2)s(-1) in the bilayer regions and approximate to 0.01 mu m(2) s(-1) in the monolayer regions. This order of magnitude difference in diffusion coefficients effectively serves to isolate the bilayer regions from one another, thus creating a bilayer array.
机译：这项工作证明了可光裂解的胆固醇衍生物在二氧化硅上产生支持的双层脂质膜阵列的用途。通过使用胺官能化的自组装单分子层（SAM）和基于N-羟基琥珀酰亚胺的试剂9的反应，将可光裂解的胆固醇酯系链连接到表面。所得的SAM包含邻硝基苄基残基，该残基可通过使用光解来裂解柔和的（365 nm）紫外线可再生原始胺表面，并且可以使用掩模进行图案化。光反应产率约为75％，这比先前在金基底上进行相关的邻硝基苄基光化学发现的产率高得多。通过接触角测量，椭圆偏振和X射线光电子能谱对SAM进行表征。用SEM和AFM表征图案化的表面。将图案化的表面浸入包含卵磷脂酰胆碱（PC）的小单层囊泡的溶液中后，形成了支持的脂质膜，该膜由胺官能化的“亲水”区域上的脂质双层和胆固醇胆固醇的“疏水”区域上的脂质单层组成。通过荧光显微镜和AFM证实了这一点。 FRAP研究在双层区域中产生的探针分子横向扩散系数为0.14 +/- 0.05μm（2）s（-1），在单层区域中约为0.01μm（2）s（-1）。扩散系数的这种数量级差异有效地用于将双层区域彼此隔离，从而创建双层阵列。

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