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首页> 外文期刊>Taiwanese journal of obstetrics and gynecology >Genetic Network Analysis of Human CD34~+ Hematopoietic Stem/Precursor Cells
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Genetic Network Analysis of Human CD34~+ Hematopoietic Stem/Precursor Cells

机译：人CD34〜+造血干/前体细胞的遗传网络分析

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Objective: Somatic CD34~+ hematopoietic stem/precursor cells (HSPCs) give rise to hematopoietic cells and endothelial cells and have been used in clinical applications. Understanding the genes responsible for sternness and how they interact with each other will help us to manipulate these cells more efficiently in the future. Materials and Methods: We performed microarray analysis on human CD34~+ HSPCs and on two different progeny cell types, i.e. microvascular endothelial cells and peripheral blood mononuclear cells. Systems biology and advanced bioinformatics tools were used to help clarify the genetic networks associated with these stem cell genes.Results: We identified CD34~+ HSPC genes and found that they were involved in critical biologic processes such as cell cycle regulation, chromosome organization, and DNA repair. We also identified a novel precursor gene cluster on chromosome 19p13.3. Analysis of HSPC-enriched genes using systems biology tools revealed a complex genetic network functioning in CD34~+ cells, in which several genes acted as hubs to maintain the stability (such as CATA1) or connectivity (such as hepatic growth factor) of the whole network.Conclusion: This study provides the foundation for a more detailed understanding of CD34~+ HSPCs.
机译：目的：体细胞CD34〜+造血干/前体细胞（HSPC）产生造血细胞和内皮细胞，并已用于临床。了解造成严峻性的基因以及它们如何相互作用将有助于我们将来更有效地操纵这些细胞。材料和方法：我们对人CD34〜+ HSPCs和两种不同的子代细胞类型，即微血管内皮细胞和外周血单核细胞进行了微阵列分析。结果：我们鉴定了CD34〜+ HSPC基因，发现它们参与了关键的生物学过程，例如细胞周期调控，染色体组织和细胞分裂，并利用系统生物学和先进的生物信息学工具来阐明与这些干细胞基因相关的遗传网络。 DNA修复。我们还在染色体19p13.3上鉴定了一个新的前体基因簇。使用系统生物学工具对富含HSPC的基因进行分析后，发现在CD34〜+细胞中有一个复杂的遗传网络，其中几个基因充当维持整个系统稳定性（例如CATA1）或连通性（例如肝生长因子）的枢纽。结论：本研究为更详细地了解CD34〜+ HSPC提供了基础。

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