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首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Biocompatible core-shell nanoparticle-based surface-enhanced Raman scattering probes for detection of DNA related to HIV gene using silica-coated magnetic nanopartieles as separation tools
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Biocompatible core-shell nanoparticle-based surface-enhanced Raman scattering probes for detection of DNA related to HIV gene using silica-coated magnetic nanopartieles as separation tools

机译：基于生物相容性核壳纳米粒子的表面增强拉曼散射探针，用于检测与HIV基因相关的DNA，使用涂有二氧化硅的磁性纳米粒子作为分离工具

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摘要

A novel,highly selective DNA hybridization assay has been developed based on surface-enhanced Raman scattering (SERS) for DNA sequences related to HIV.This strategy employs the Ag/SiO2 core-shell nanoparticle-based Raman tags and the amino group modified silica-coated magnetic nanopartieles as immobilization matrix and separation tool.The hybridization reaction was performed between Raman tags functionalized with 3'-amino-labeled oligonucleotides as detection probes and the amino group modified silica-coated magnetic nanopartieles functionalized with 5'-amino-labeled oligonucleotides as capture probes.The Raman spectra of Raman tags can be used to monitor the presence of target oligonucleotides.The utilization of silica-coated magnetic nanopartieles not only avoided time-consuming washing,but also amplified the signal of hybridization assay.Additionally,the results of control experiments show that no or very low signal would be obtained if the hybridization assay is conducted in the presence of DNA sequences other than complementary oligonucleotides related to HIV gene such as non-complementary oligonucleotides,four bases mismatch oligonucleotides,two bases mismatch oligonucleotides and even single base mismatch oligonucleotides.It was demonstrated that the method developed in this work has high selectivity and sensitivity for DNA detection related to HIV gene.
机译：已开发出一种基于表面增强拉曼散射（SERS）的新型高选择性DNA杂交测定方法，用于检测与HIV相关的DNA序列。该策略采用基于Ag / SiO2核壳纳米粒子的拉曼标签和氨基修饰的二氧化硅-以3'-氨基标记的寡核苷酸为检测探针功能化的拉曼标签和以5'-氨基标记的寡核苷酸为功能化的氨基修饰的二氧化硅包覆的磁性纳米粒子之间的杂交反应。 Raman标签的Raman光谱可用于监测目标寡核苷酸的存在。利用二氧化硅包被的磁性纳米粒子的使用不仅避免了费时的洗涤，而且放大了杂交测定的信号。对照实验表明，如果在原位杂交中进行杂交测定，则不会获得或非常低的信号与HIV基因相关的互补寡核苷酸以外的DNA序列的实质，例如非互补寡核苷酸，四碱基错配寡核苷酸，两碱基错配寡核苷酸，甚至单碱基错配寡核苷酸。证明了该方法开发的方法具有很高的选择性和灵敏度。用于检测与HIV基因相关的DNA。

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