• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Preparation of a strong-cation exchange monolith by a novel method and its application in the separation of IgG on high performance liquid chromatography
【24h】

Preparation of a strong-cation exchange monolith by a novel method and its application in the separation of IgG on high performance liquid chromatography

机译：一种新型强阳离子交换整体柱的制备及其在高效液相色谱分离IgG中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A strong cation-exchange poly(vinyl carboxylate-co-ethyleneglycol dimethacrylate) (poly(VC-co-EDMA)) monolithic column for high performance liquid chromatography (HPLC) has been prepared firstly by atom transfer radical polymerization (ATRP) without the expensive complexing ligand, in which vinyl carboxylate was used as the monomer, ethyleneglycol dimethacrylate as the cross linking agent, carbon tetrachloride as the initiator and ferrous chloride as the catalyst. Conditions of the polymerization have been studied and optimized. Morphology of monolithic materials was studied by scanning electronic microscopy. Chemical groups of the monolith were assayed by infrared spectra method and the pore size distribution was determined by a mercury porosimeter. Moreover, the monolith was modified to bear strong-cation exchange groups and tested on the separation of human immune globulin G (IgG) from human plasma in conjunction with HPLC. Good resolution was obtained in a short time (10 min) in the separation. The effects of pH and buffer concentration on the elution of IgG have been investigated. Moreover, frontal analytical method was used to get the IgG dynamic banding capacity of the monolith that was 3.0 mg g~(-1). Besides, the monolith was also used to separate lysozyme from egg white and separate the mixture of papain, snailase and IgG.
机译：首先通过原子转移自由基聚合（ATRP）制备了不昂贵的强阳离子交换聚羧酸乙烯酯-乙二醇二甲基丙烯酸乙二醇酯（VC-co-EDMA）整体柱，用于高效液相色谱（HPLC）络合配体，其中羧酸乙烯酯为单体，乙二醇二甲基丙烯酸酯为交联剂，四氯化碳为引发剂，氯化亚铁为催化剂。已经研究和优化了聚合条件。通过扫描电子显微镜研究了整体材料的形态。通过红外光谱法测定整料的化学基团，并通过水银孔率计测定孔径分布。此外，整料经过修饰以带有强阳离子交换基团，并结合HPLC对人免疫球蛋白G（IgG）从人血浆中的分离进行了测试。在短时间内（10分钟）分离获得了良好的分离度。研究了pH和缓冲液浓度对IgG洗脱的影响。此外，采用额叶分析法得到的整体柱IgG动态条带容量为3.0 mg g〜（-1）。此外，整料还用于从蛋清中分离溶菌酶，并分离木瓜蛋白酶，蜗牛酶和IgG的混合物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号