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首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Indirect method for spectrophotometric determination of ascorbic acid in pharmaceutical preparations with 2,4,6-tripyridyl-s-triazine by flow-injection analysis
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Indirect method for spectrophotometric determination of ascorbic acid in pharmaceutical preparations with 2,4,6-tripyridyl-s-triazine by flow-injection analysis

机译：流动注射分析法间接测定2,4,6-三吡啶基-s-三嗪分光光度法测定药物制剂中的抗坏血酸

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摘要

A flow-injection indirect spectrophotometric method for the determination of ascorbic acid (AA) in pharmaceutical preparations is proposed. The method is based on the reduction of iron(III) to iron(II) by the AA, and by the subsequent reaction of the produced iron(II) with 2,4,6-tripyridyl-s-triazine (TPTZ) in buffered medium (pH = 3.6) to form a coloured complex (λ _(max) = 593 nm). The three-line manifold with one reaction coil was used. The linear range of the method is from 0.08 to 10 μM of ascorbic acid, with the detection limit 24 nM of AA. The proposed method is simple, rapid (sampling rate of 180 samples per hour), sensitive and reproducible (RSD 0.8%, n = 100). The proposed method is very selective, because only the reducing substances with standard (formal) potentials lower than 0.6 V would have the thermodynamic predisposition to interfere in the proposed method. Tested reducing substances (thiol compounds) did not give serious errors when present at the same concentrations as the ascorbic acid. The proposed method can be applied for the determination of AA in pharmaceutical preparations, down to picomolar quantity.
机译：提出了一种流动注射间接分光光度法测定药物制剂中抗坏血酸的方法。该方法基于AA将铁（III）还原为铁（II），以及随后在缓冲液中将生成的铁（II）与2,4,6-三吡啶基-s-三嗪（TPTZ）反应介质（pH = 3.6）形成有色络合物（λ_（max）= 593 nm）。使用带有一个反应线圈的三线歧管。该方法的线性范围为0.08至10μM的抗坏血酸，检测限为24 nM AA。所提出的方法简单，快速（每小时180个样品的采样率），灵敏且可重现（RSD 0.8％，n = 100）。所提出的方法非常有选择性，因为只有标准（形式）电势低于0.6 V的还原性物质才会具有热力学倾向来干扰所提出的方法。当以与抗坏血酸相同的浓度存在时，经测试的还原性物质（硫醇化合物）不会产生严重的错误。所提出的方法可用于测定药物制剂中的AA（低至皮摩尔量）。

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