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Optical Sensors Based on Hybrid DNA/Conjugated Polymer Complexes

机译:基于杂化DNA /共轭聚合物复合物的光学传感器

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摘要

Single-stranded DNA (ss-DNA) can specifically bind to various targets,including a complementary ss-DNA,ions,proteins,drugs,and so forth.When binding takes place,the oligonucleotide probe often undergoes a conformational transition.This conformational change of the negatively charged ss-DNA can be detected by using a water-soluble,cationic polythiophene derivative,which transduces the complex formation into an optical (colorimetric or fluorometric) signal without any labeling of the probe or the target.This simple and rapid methodology has enabled the specific and sensitive detection of nucleic acids and human thrombin.This new biophotonic tool can easily be applied to the detection of various other biomolecules and is also useful in the high-throughput screening of new drugs.
机译:单链DNA(ss-DNA)可以特异性结合各种靶标,包括互补的ss-DNA,离子,蛋白质,药物等。发生结合时,寡核苷酸探针通常会发生构象转变。这种构象变化可以通过使用水溶性阳离子聚噻吩衍生物检测带负电荷的ss-DNA的方法,该衍生物将复合物的形成转换为光学(比色或荧光)信号,而无需对探针或靶标进行任何标记。能够对核酸和人类凝血酶进行特异性和灵敏的检测。这种新的生物光子工具可以轻松地用于检测其他各种生物分子,也可用于高通量筛选新药。

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