• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Talanta: The International Journal of Pure and Applied Analytical Chemistry >Operator care and eco-concerned development of a fast, facile and economical assay for basic nitrogenous drugs based on simplified ion-pair mini-scale extraction using safer solvent combined with drop-based spectrophotometry
【24h】

Operator care and eco-concerned development of a fast, facile and economical assay for basic nitrogenous drugs based on simplified ion-pair mini-scale extraction using safer solvent combined with drop-based spectrophotometry

机译：基于安全的溶剂与基于液滴的分光光度法结合的简化的离子对小规模萃取，基于操作人员的关心和对生态的关注，开发了一种快速，简便且经济的基本含氮药物测定方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A fast, facile, and economical assay for basic nitrogenous drugs has been developed based on the mini-scale extraction of the drug-dye ion pair complex combined with the use of safe-for-analyst and eco-friendlier organic extractant and drop-based micro-spectrophotometry. Instead of using large volume devices, the extraction was simply carried out in typical 1.5 mL microcentrifuge tubes along with the use of micropipettes for accurate transfer of liquids, vortex mixer for efficient partitioning of solutes and benchtop centrifuge for rapid phase separation. In the last step, back-extraction was performed by using the microvolume of acidic solution in order to concentrate the colored species into a confined aqueous microdrop and to keep the analyst away from unwanted contact and inhalation of organic solvents during the quantitation step which was achieved by using cuvetteless UV-vis micro-spectrophotometry without any prior dilutions. Using chlorpheniramine maleate as a representative analyte and n-butyl acetate as a less toxic and non-ozone depleting extractant, the miniaturized method was less laborious and much faster. It was accurate, precise and insensitive to the interferences from common excipients. Notably, it gave the assay results of drug in tablets and oral solution comparable to the large-scale pharmacopeial method while the consumption of organic solvents and the release of wastes were lowered by 200-400 folds.
机译：基于微量的药物-染料离子对复合物的提取，并结合使用安全分析和生态友好的有机提取剂和基于滴剂的方法，开发了一种快速，简便且经济的基本含氮药物测定方法显微分光光度法。无需使用大容量设备，只需在典型的1.5 mL微量离心管中进行提取即可，同时使用微量移液器进行液体的准确转移，使用涡旋混合器进行有效的溶质分配，并使用台式离心机进行快速的相分离。在最后一步中，通过使用微体积的酸性溶液进行反萃取，以将有色物质浓缩到密闭的水微滴中，并在完成定量步骤的过程中使分析人员远离有害的接触和有机溶剂的吸入通过使用无比色皿的紫外可见微分光光度法，无需事先稀释。使用马来酸氯苯那敏作为代表性分析物，使用乙酸正丁酯作为毒性较小且不消耗臭氧的萃取剂，微型化方法省时省力，而且速度更快。它对普通赋形剂的干扰是准确，精确和不敏感的。值得注意的是，它使片剂和口服溶液中的药物测定结果与大规模药典法相当，而有机溶剂的消耗和废物的释放降低了200-400倍。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号