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首页> 外文期刊>Chemistry: A European journal >Induced-Fit Recognition of DNA by Small Circular Oligonucleotides
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Induced-Fit Recognition of DNA by Small Circular Oligonucleotides

机译：小环状寡核苷酸对DNA的诱导拟合识别

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摘要

We have investigated the molecular interaction between cyclic and linear oligonucleotides.We have found that short cyclic oligonucleotides can induce hairpinlike structures in linear DNA fragments.By using NMR and CD spectroscopy we have studied the interaction of the cyclic oligonucleotide dCCTTCGGT> with d < pCAGTCCCT >,as well as with its two linear analogs d(GTCCCTCA)and d(CTCAGTCC).Here we report the NMR structural study of these complexes.Recognition between these oligonucleotides occurs through formation of four intermolecular Watson-Crick base pairs.The three-dimensional structure is stabilized by two tetrads,formed by facing the minor-groove side of the Watson-Crick base pairs.Overall,the structure is similar to those observed previously in other quadruplex-es formed by minor-groove alignment of Watson-Crick base pairs.However,in this case the complexes are heterodi-meric and are formed by two different tetrads(G:C:A:T and G:C:G:C).These complexes represent a new model of DNA recognition by small cyclic oligonucleotides,increasing the number of potential applications of these interesting molecules.
机译：我们研究了环状和线性寡核苷酸之间的分子相互作用，发现短环状寡核苷酸可以诱导线性DNA片段中的发夹状结构。通过NMR和CD光谱，我们研究了环状寡核苷酸d CCTTCGGT>与d < pCAGTCCCT>及其两个线性类似物d（GTCCCTCA）和d（CTCAGTCC）。在此我们报告了这些复合物的NMR结构研究。这些寡核苷酸之间的识别是通过形成四个分子间Watson-Crick碱基对实现的。二维结构通过面对沃森-克里克碱基对的次要沟槽侧面而形成的两个四元体得以稳定。总体而言，该结构与先前在其他通过沃森-克里克的次要沟槽排列形成的四链体中观察到的结构相似但是，在这种情况下，复合物是异二聚体，由两个不同的四元组（G：C：A：T和G：C：G：C）形成。这些复合物代表了DN的新模型小环状寡核苷酸的识别，增加了这些有趣分子的潜在应用数量。

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