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首页> 外文期刊>Canadian Journal of Zoology >Molluscan cells in culture: primary cell cultures and cell lines1
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Molluscan cells in culture: primary cell cultures and cell lines1

机译：培养中的软体动物细胞：原代细胞培养和细胞系1

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摘要

In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata (Say, 1818) embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell culturesin research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host - parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.
机译：软体动物的体外细胞培养系统极大地促进了我们对组织特异性细胞内或组织之间发生的复杂生理过程的基本了解，从而获得了使用完整动物模型无法获得的信息。腹足动物神经元细胞的体外培养表明，简化的细胞模型可如何帮助我们理解完整生物中的复杂网络。海洋和淡水双壳类和腹足类物种的原代细胞培养物被用作环境污染物的生物监测器，基因转移技术的模型以及先天免疫和肿瘤性疾病的研究。尽管努力从软体动物中分离增殖性细胞系，但蜗牛Biomphalaria glabrata（Say，1818）胚胎（Bge）细胞系是唯一现存的源自任何软体动物物种的细胞系。本文采用器官系统方法，总结了建立软体动物细胞培养物的努力，并描述了原代细胞培养物在研究中的各种应用。由于Bge细胞系的独特状态，因此介绍了该细胞系的建立以及这些细胞如何促进我们对蜗牛宿主-寄生虫相互作用的理解。最后，我们详细介绍了从软体动物建立细胞系时通常遇到的困难，并讨论了如何克服这些困难。

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